Developing ROR1 Targeting CAR-T Cells against Solid Tumors in Preclinical Studies
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Lines
2.2. CAR Constructs and Lentivirus Preparation
2.3. Generation of ROR-1 CAR-T
2.4. LDH Assay
2.5. Flow Cytometry
2.6. Animal Protocol
2.7. Statistical Analyses
3. Results
3.1. ROR1 Is Expressed on Various Human Tumor Cell Lines
3.2. ROR1 CAR with Shorter Spacer Region Exhibited Superior Expression
3.3. ROR1 Hinge CAR-T-Cells Demonstrated Potent Anti-Tumor Activities
3.4. ROR1 Hinge CAR Cells also Demonstrated Promising Effect on Murine Solid Tumor Models
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lee, B.k.; Wan, Y.; Chin, Z.l.; Deng, L.; Deng, M.; Leung, T.m.; Hua, J.; Zhang, H. Developing ROR1 Targeting CAR-T Cells against Solid Tumors in Preclinical Studies. Cancers 2022, 14, 3618. https://doi.org/10.3390/cancers14153618
Lee Bk, Wan Y, Chin Zl, Deng L, Deng M, Leung Tm, Hua J, Zhang H. Developing ROR1 Targeting CAR-T Cells against Solid Tumors in Preclinical Studies. Cancers. 2022; 14(15):3618. https://doi.org/10.3390/cancers14153618
Chicago/Turabian StyleLee, Boon kiat, Yuhua Wan, Zan lynn Chin, Linyan Deng, Mo Deng, Tze ming Leung, Jian Hua, and Hua Zhang. 2022. "Developing ROR1 Targeting CAR-T Cells against Solid Tumors in Preclinical Studies" Cancers 14, no. 15: 3618. https://doi.org/10.3390/cancers14153618