Brassinin Promotes the Degradation of Tie2 and FGFR1 in Endothelial Cells and Inhibits Triple-Negative Breast Cancer Angiogenesis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Chemicals
2.3. Cell Culture
2.4. Water-Soluble Tetrazolium (WST)-1 Assay
2.5. Lactate Dehydrogenase (LDH) Assay
2.6. Bromodeoxyuridine (BrdU) Incorporation Assay
2.7. Transwell Migration Assay
2.8. Tube Formation Assay
2.9. Spheroid Sprouting Assay
2.10. Aortic Ring Assay
2.11. Animal Models
2.12. Histology and Immunohistochemistry
2.13. Western Blotting
2.14. Quantitative Real-Time Polymerase Chain Reaction (PCR)
2.15. Statistics
3. Results
3.1. Brassinin Preferentially Targets ECs
3.2. Brassinin Suppresses the Angiogenic Activity of ECs
3.3. Brassinin Inhibits Angiogenesis Ex Vivo and In Vivo
3.4. Brassinin Inhibits Tumor Growth and Angiogenesis
3.5. Brassinin Down-Regulates Key Angiogenic Signaling Pathways
3.6. Brassinin Promotes Tie2 and FGFR1 Degradation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gu, Y.; Becker, V.; Qiu, M.; Tang, T.; Ampofo, E.; Menger, M.D.; Laschke, M.W. Brassinin Promotes the Degradation of Tie2 and FGFR1 in Endothelial Cells and Inhibits Triple-Negative Breast Cancer Angiogenesis. Cancers 2022, 14, 3540. https://doi.org/10.3390/cancers14143540
Gu Y, Becker V, Qiu M, Tang T, Ampofo E, Menger MD, Laschke MW. Brassinin Promotes the Degradation of Tie2 and FGFR1 in Endothelial Cells and Inhibits Triple-Negative Breast Cancer Angiogenesis. Cancers. 2022; 14(14):3540. https://doi.org/10.3390/cancers14143540
Chicago/Turabian StyleGu, Yuan, Vivien Becker, Moqin Qiu, Tianci Tang, Emmanuel Ampofo, Michael D. Menger, and Matthias W. Laschke. 2022. "Brassinin Promotes the Degradation of Tie2 and FGFR1 in Endothelial Cells and Inhibits Triple-Negative Breast Cancer Angiogenesis" Cancers 14, no. 14: 3540. https://doi.org/10.3390/cancers14143540