LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Database and Online Tools
2.2. Cell Culture
2.3. Cell Transfection
2.4. Quantitative Real-Time PCR (qRT-PCR)
2.5. Luciferase Reporter Assays
2.6. CCK-8 Assay
2.7. Colony Formation Assay
2.8. Western Blot
2.9. Statistical Analysis
3. Results
3.1. HOXC-AS1 Was Overexpressed in GC with Poor Prognosis
3.2. Downregulation of HOXC-AS1 Inhibited GC Cell Proliferation
3.3. HOXC-AS1 Sponged miR-99a-3p in GC
3.4. MiR-99a-3p Regulated MMP8 in GC
3.5. HOXC-AS1 Promoted GC by Managing miR-99a-3p/MMP8 Axis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jiang, Y.; Li, X.; Yang, Y.; Luo, J.; Ren, X.; Yuan, J.; Tong, Q. LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer. Cancers 2022, 14, 3534. https://doi.org/10.3390/cancers14143534
Jiang Y, Li X, Yang Y, Luo J, Ren X, Yuan J, Tong Q. LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer. Cancers. 2022; 14(14):3534. https://doi.org/10.3390/cancers14143534
Chicago/Turabian StyleJiang, Yue, Xiangpan Li, Yu Yang, Jiajun Luo, Xunshan Ren, Jingwen Yuan, and Qiang Tong. 2022. "LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer" Cancers 14, no. 14: 3534. https://doi.org/10.3390/cancers14143534