Next Article in Journal
Editorial on Special Issue “Immunotherapy, Tumor Microenvironment and Survival Signaling”
Next Article in Special Issue
Skeletal Muscle Depletion and Major Postoperative Complications in Locally-Advanced Head and Neck Cancer: A Comparison between Ultrasound of Rectus Femoris Muscle and Neck Cross-Sectional Imaging
Previous Article in Journal
Prognostic and Therapeutic Implications of Tumor Biology in Colorectal Liver Metastases
Previous Article in Special Issue
Bile Acid Dysregulation Is Intrinsically Related to Cachexia in Tumor-Bearing Mice

Aging Aggravates Cachexia in Tumor-Bearing Mice

Institute for Diabetes and Cancer, Helmholtz Center Munich, 85764 Neuherberg, Germany
Joint Heidelberg-IDC Translational Diabetes Program, Inner Medicine 1, Heidelberg University Hospital, 69120 Heidelberg, Germany
German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany
Institute for Diabetes Research, Research Group Immune Tolerance in Diabetes, Helmholtz Diabetes Center at Helmholtz Center Munich, 85764 Neuherberg, Germany
Department of Surgery and LIM 26, Faculdade de Medicina, University of Sao Paulo, Sao Paulo 01246-903, Brazil
Division of Clinical Pharmacology, Department of Medicine IV, Ludwig-Maximilians-Universität, 80539 Munich, Germany
Chair Molecular Metabolic Control, TUM School of Medicine, Faculty of Medicine, Technical University Munich, 80333 Munich, Germany
Author to whom correspondence should be addressed.
Academic Editor: Tateaki Naito
Cancers 2022, 14(1), 90;
Received: 20 October 2021 / Revised: 17 December 2021 / Accepted: 20 December 2021 / Published: 24 December 2021
(This article belongs to the Special Issue Cancer-Associated Cachexia)
Cachexia is a deadly disease that accompanies many different types of cancers. Animal studies on cachexia have so far mostly been conducted using young mice, while cancer in humans is a disease of high age. Mouse models used to date may therefore not be suitable to study cachexia with relevance to patients. By comparing young and old mice of three different strains and two different tumor types, we here show that the age of mice has a substantial effect on cachexia progression (specifically body weight, tissue weight, fiber size, molecular markers) that is dependent on the mouse strain studied. This is independent of glucose tolerance. The cachexia markers IL6 and GDF15 differ between ages in both mice and patients. Future studies on cachexia should consider the age and strain of mice.
Background: Cancer is primarily a disease of high age in humans, yet most mouse studies on cancer cachexia are conducted using young adolescent mice. Given that metabolism and muscle function change with age, we hypothesized that aging may affect cachexia progression in mouse models. Methods: We compare tumor and cachexia development in young and old mice of three different strains (C57BL/6J, C57BL/6N, BALB/c) and with two different tumor cell lines (Lewis Lung Cancer, Colon26). Tumor size, body and organ weights, fiber cross-sectional area, circulating cachexia biomarkers, and molecular markers of muscle atrophy and adipose tissue wasting are shown. We correlate inflammatory markers and body weight dependent on age in patients with cancer. Results: We note fundamental differences between mouse strains. Aging aggravates weight loss in LLC-injected C57BL/6J mice, drives it in C57BL/6N mice, and does not influence weight loss in C26-injected BALB/c mice. Glucose tolerance is unchanged in cachectic young and old mice. The stress marker GDF15 is elevated in cachectic BALB/c mice independent of age and increased in old C57BL/6N and J mice. Inflammatory markers correlate significantly with weight loss only in young mice and patients. Conclusions: Aging affects cachexia development and progression in mice in a strain-dependent manner and influences the inflammatory profile in both mice and patients. Age is an important factor to consider for future cachexia studies. View Full-Text
Keywords: aging; cachexia; cancer; mouse models aging; cachexia; cancer; mouse models
Show Figures

Figure 1

MDPI and ACS Style

Geppert, J.; Walth, A.A.; Terrón Expósito, R.; Kaltenecker, D.; Morigny, P.; Machado, J.; Becker, M.; Simoes, E.; Lima, J.D.C.C.; Daniel, C.; Berriel Diaz, M.; Herzig, S.; Seelaender, M.; Rohm, M. Aging Aggravates Cachexia in Tumor-Bearing Mice. Cancers 2022, 14, 90.

AMA Style

Geppert J, Walth AA, Terrón Expósito R, Kaltenecker D, Morigny P, Machado J, Becker M, Simoes E, Lima JDCC, Daniel C, Berriel Diaz M, Herzig S, Seelaender M, Rohm M. Aging Aggravates Cachexia in Tumor-Bearing Mice. Cancers. 2022; 14(1):90.

Chicago/Turabian Style

Geppert, Julia, Alina A. Walth, Raúl Terrón Expósito, Doris Kaltenecker, Pauline Morigny, Juliano Machado, Maike Becker, Estefania Simoes, Joanna D.C.C. Lima, Carolin Daniel, Mauricio Berriel Diaz, Stephan Herzig, Marilia Seelaender, and Maria Rohm. 2022. "Aging Aggravates Cachexia in Tumor-Bearing Mice" Cancers 14, no. 1: 90.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop