Fibroblast MMP14-Dependent Collagen Processing Is Necessary for Melanoma Growth
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Melanoma Spheroids
2.3. Tumor Grafting Experiments
2.4. Atomic Force Microscopy (AFM)
2.5. Immunoblot
2.6. Immunofluorescence Staining
2.7. Picrosirius Red Staining
2.8. Invasion Assays Using De-Epidermized Devitalized Skin (DDS)
2.9. Second Harmonic Generation
2.10. RNA Isolation and Real-Time PCR
2.11. Statistics
2.12. BrdU (5-bromo-2′-deoxyuridine) Incorporation Assay
3. Results
3.1. Reduced Melanoma Growth in MMP14Sf−/− Mice
3.2. Alterations in the Peritumoral Matrix of MMP14Sf−/− Mice
3.3. Reduced Area of Melanoma Tumor Nests in Skin Composites
3.4. High Collagen Type I and Tissue Tension Levels Negatively Modulate Melanoma Growth
3.5. Increased Tissue Stiffness and Matrix Density Levels Lead to Reduced Melanoma Growth In Vivo
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pach, E.; Brinckmann, J.; Rübsam, M.; Kümper, M.; Mauch, C.; Zigrino, P. Fibroblast MMP14-Dependent Collagen Processing Is Necessary for Melanoma Growth. Cancers 2021, 13, 1984. https://doi.org/10.3390/cancers13081984
Pach E, Brinckmann J, Rübsam M, Kümper M, Mauch C, Zigrino P. Fibroblast MMP14-Dependent Collagen Processing Is Necessary for Melanoma Growth. Cancers. 2021; 13(8):1984. https://doi.org/10.3390/cancers13081984
Chicago/Turabian StylePach, Elke, Jürgen Brinckmann, Matthias Rübsam, Maike Kümper, Cornelia Mauch, and Paola Zigrino. 2021. "Fibroblast MMP14-Dependent Collagen Processing Is Necessary for Melanoma Growth" Cancers 13, no. 8: 1984. https://doi.org/10.3390/cancers13081984