Identification and Validation of an Immune-Associated RNA-Binding Proteins Signature to Predict Clinical Outcomes and Therapeutic Responses in Glioma Patients
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Data Acquisition
2.2. Identification of Glioma Immune Subtypes Based on ssGSEA Score
2.3. Identification of Immune-Associated RBPs in Glioma
2.4. Risk-Based Modeling
2.5. Pathway Enrichment Analysis
2.6. Immuno-/Chemotherapeutic Response Prediction
2.7. Statistical Analysis
3. Results
3.1. Identification of Glioma Immune Subtypes Based on Infiltration of Immune Cells
3.2. Identification and Functional Enrichment Analysis of Immune-Associated RBPs in Glioma Patients
3.3. Identification and Assessment of an Immune-Associated RBPs Prognostic Signature for Overall Survival in Glioma.
3.4. Construction of Integrated Model to Predict Survival of Glioma Patients
3.5. Estimation of Tumor-Infiltrating Immune Cells and Prediction of Therapeutic Response to Immune Checkpoint Inhibitors
3.6. Somatic Mutation and Copy Number Variations in Different Risk Groups
3.7. GSVA and GSEA for Different Risk Groups
3.8. The Role of the Eight Immune-Associated RBPs Signature in Predicting the Sensitivity to Chemotherapeutic Agents
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Univariate Analysis | Multivariate Analysis | |||
---|---|---|---|---|
HR (95% CI) | p Value | HR (95% CI) | p Value | |
Age ≥ Median vs. < Median) | 2.891 (2.38–3.511) | <0.001 | 1.818 (1.186–2.788) | 0.006 |
Sex (Male vs. Female) | 1.189 (1.001–1.412) | 0.049 | 1.262 (0.917–1.737) | 0.154 |
Grade (WHO IV vs. WHO II~III) | 4.729 (3.913–5.716) | <0.001 | 1.402 (0.904–2.173) | 0.131 |
ATRX.status (WT vs. Mutant) | 1.911 (1.528–2.389) | <0.001 | 1.095 (0.623–1.924) | 0.754 |
IDH.status (WT vs. Mutant) | 5.032 (4.12–6.146) | <0.001 | 2.28 (1.256–4.139) | 0.007 |
MGMT.promoter (Methylated vs. Unmethylated) | 2.333 (1.924–2.829) | <0.001 | 0.854 (0.573–1.272) | 0.436 |
TERT.promoter (WT vs. Mutant) | 0.588 (0.44–0.785) | <0.001 | 0.948 (0.558–1.612) | 0.845 |
Immune subtype (Sub2 vs. Sub1) | 3.262 (2.708–3.929) | <0.001 | 1.414 (0.912–2.195) | 0.122 |
Risk (High vs. Low) | 4.335 (3.468–5.421) | <0.001 | 1.897 (1.147–3.138) | 0.013 |
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Tian, R.; Li, Y.; Liu, Q.; Shu, M. Identification and Validation of an Immune-Associated RNA-Binding Proteins Signature to Predict Clinical Outcomes and Therapeutic Responses in Glioma Patients. Cancers 2021, 13, 1730. https://doi.org/10.3390/cancers13071730
Tian R, Li Y, Liu Q, Shu M. Identification and Validation of an Immune-Associated RNA-Binding Proteins Signature to Predict Clinical Outcomes and Therapeutic Responses in Glioma Patients. Cancers. 2021; 13(7):1730. https://doi.org/10.3390/cancers13071730
Chicago/Turabian StyleTian, Ruotong, Yimin Li, Qian Liu, and Minfeng Shu. 2021. "Identification and Validation of an Immune-Associated RNA-Binding Proteins Signature to Predict Clinical Outcomes and Therapeutic Responses in Glioma Patients" Cancers 13, no. 7: 1730. https://doi.org/10.3390/cancers13071730