Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Lines and Culture Conditions
2.2. Spheroid Formation Assay
2.3. siRNA Transfections and Western Blots
2.4. RNA Extraction and Quantitative Reverse Transcription PCR (qRT-PCR)
2.5. Cell Viability Assay
2.6. Flow Cytometry
2.7. Animal Experiments
2.8. Immunohistochemistry (IHC)
2.9. Public Database Analysis
2.10. Statistical Analysis
2.11. Data Availability
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Robinson, M.; Gilbert, S.F.; Waters, J.A.; Lujano-Olazaba, O.; Lara, J.; Alexander, L.J.; Green, S.E.; Burkeen, G.A.; Patrus, O.; Sarwar, Z.; et al. Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells. Cancers 2021, 13, 262. https://doi.org/10.3390/cancers13020262
Robinson M, Gilbert SF, Waters JA, Lujano-Olazaba O, Lara J, Alexander LJ, Green SE, Burkeen GA, Patrus O, Sarwar Z, et al. Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells. Cancers. 2021; 13(2):262. https://doi.org/10.3390/cancers13020262
Chicago/Turabian StyleRobinson, Mikella, Samuel F. Gilbert, Jennifer A. Waters, Omar Lujano-Olazaba, Jacqueline Lara, Logan J. Alexander, Samuel E. Green, Gregory A. Burkeen, Omid Patrus, Zinia Sarwar, and et al. 2021. "Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells" Cancers 13, no. 2: 262. https://doi.org/10.3390/cancers13020262