Overexpression of Replication-Dependent Histone Signifies a Subset of Dedifferentiated Liposarcoma with Increased Aggressiveness
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Subjects and Tumor Samples
2.2. Tumor Cell Indexing
2.3. Whole-Exome Sequencing
2.4. Bulk and Single-Cell RNA-Sequencing
2.5. LPS Cell Lines and Patient-Derived 18DDLPS
2.6. Western Blots for Fresh, Frozen, and FFPE Tissues
2.7. Immunohistochemistry
2.8. Lentivirus Production and Transduction
2.9. Western Blots for HMGA2 Knockdown and Overexpression
2.10. Colony Forming Assay
2.11. Mouse Xenograft Model and Subcutaneous Transplants
2.12. Cell Viability Assays
2.13. Real-Time Quantitative PCR (RT-qPCR)
2.14. Statistical Analyses
3. Results
3.1. Comparison of WD- and DDLPS Expression Profiles
3.2. Overexpression of Replication-Dependent Histone mRNAs in DDLPS
3.3. Overexpression of HMGA2 by the SCNAs
3.4. HMGA2 Regulated RD-HISTs’ Expression
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yoo, Y.; Park, S.-Y.; Jo, E.B.; Choi, M.; Lee, K.W.; Hong, D.; Lee, S.; Lee, C.-R.; Lee, Y.; Um, J.-Y.; et al. Overexpression of Replication-Dependent Histone Signifies a Subset of Dedifferentiated Liposarcoma with Increased Aggressiveness. Cancers 2021, 13, 3122. https://doi.org/10.3390/cancers13133122
Yoo Y, Park S-Y, Jo EB, Choi M, Lee KW, Hong D, Lee S, Lee C-R, Lee Y, Um J-Y, et al. Overexpression of Replication-Dependent Histone Signifies a Subset of Dedifferentiated Liposarcoma with Increased Aggressiveness. Cancers. 2021; 13(13):3122. https://doi.org/10.3390/cancers13133122
Chicago/Turabian StyleYoo, Yongjin, Sang-Yoon Park, Eun Byeol Jo, Minji Choi, Kyo Won Lee, Doopyo Hong, Sangmoon Lee, Cho-Rong Lee, Youngha Lee, Jae-Young Um, and et al. 2021. "Overexpression of Replication-Dependent Histone Signifies a Subset of Dedifferentiated Liposarcoma with Increased Aggressiveness" Cancers 13, no. 13: 3122. https://doi.org/10.3390/cancers13133122