CCR2 of Tumor Microenvironmental Cells Is a Relevant Modulator of Glioma Biology
Abstract
:1. Introduction
2. Results
2.1. CCR2-Expression of Microglia/Macrophages in Human and Murine Glioblastoma Tissue
2.2. Diminished Infiltration of TAMs in Brain Tumor Tissues of Ccr2KO Mice
2.3. Ccr2-Deficiency Leads to Accelerated Brain Tumor Growth
2.4. TAMs of Ccr2KO Mice Show Classical Markers but Exhibit a Modified Expression of Inflammatory Genes
2.5. Improved Vascular Integrity in Ccr2-Deficient Mice
3. Discussion
4. Materials and Methods
4.1. Human Specimens
4.2. Animals
4.3. Intracerebral Tumor Cell Inoculation
4.4. MRI Analysis
4.5. Lectin Application
4.6. Cardial Brain Perfusion
4.7. Immunohistochemistry
4.8. Immunofluorescence Staining
4.9. Cell Homogenization
4.10. Isolation of CD11b+ Cells
4.11. Isolation of Bone Marrow Cells and Generation of Macrophages
4.12. RNA Isolation Real-Time PCRs
4.13. Culture of Myeloid Cells In Vitro and Immunocytochemistry
4.14. Flow Cytometry
4.15. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Felsenstein, M.; Blank, A.; Bungert, A.D.; Mueller, A.; Ghori, A.; Kremenetskaia, I.; Rung, O.; Broggini, T.; Turkowski, K.; Scherschinski, L.; et al. CCR2 of Tumor Microenvironmental Cells Is a Relevant Modulator of Glioma Biology. Cancers 2020, 12, 1882. https://doi.org/10.3390/cancers12071882
Felsenstein M, Blank A, Bungert AD, Mueller A, Ghori A, Kremenetskaia I, Rung O, Broggini T, Turkowski K, Scherschinski L, et al. CCR2 of Tumor Microenvironmental Cells Is a Relevant Modulator of Glioma Biology. Cancers. 2020; 12(7):1882. https://doi.org/10.3390/cancers12071882
Chicago/Turabian StyleFelsenstein, Matthäus, Anne Blank, Alexander D. Bungert, Annett Mueller, Adnan Ghori, Irina Kremenetskaia, Olga Rung, Thomas Broggini, Kati Turkowski, Lea Scherschinski, and et al. 2020. "CCR2 of Tumor Microenvironmental Cells Is a Relevant Modulator of Glioma Biology" Cancers 12, no. 7: 1882. https://doi.org/10.3390/cancers12071882