Next Article in Journal
Molecular Pathways and Targeted Therapies for Malignant Ovarian Germ Cell Tumors and Sex Cord–Stromal Tumors: A Contemporary Review
Next Article in Special Issue
Nanomedicine in Non-Small Cell Lung Cancer: From Conventional Treatments to Immunotherapy
Previous Article in Journal
Mechanisms of B Cell Receptor Activation and Responses to B Cell Receptor Inhibitors in B Cell Malignancies
Previous Article in Special Issue
Nanoemulsions as Delivery Systems for Poly-Chemotherapy Aiming at Melanoma Treatment
Article

Tailored Functionalized Magnetic Nanoparticles to Target Breast Cancer Cells Including Cancer Stem-Like Cells

1
Departamento de Biología, Universidad Autónoma de Madrid, Darwin 2, 28049 Madrid, Spain
2
Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA Nanociencia), Faraday 9, 28049 Madrid, Spain
3
Departamento Química Física, Universidad Complutense de Madrid, Avda. Séneca 2, 28040 Madrid, Spain
4
Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramón 182, 20014 Donostia San Sebastián, Spain
5
Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain
6
Breast Cancer Now Research Unit, Division of Cancer Sciences, University of Manchester, Manchester Cancer Research Centre, 555 Wilmslow Road, Manchester M20 4GJ, UK
7
Departamento de Física de la Materia Condensada, Universidad Autónoma Madrid, Francisco Tomás y Valiente 7, 28049 Madrid, Spain
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(6), 1397; https://doi.org/10.3390/cancers12061397
Received: 7 May 2020 / Revised: 25 May 2020 / Accepted: 27 May 2020 / Published: 29 May 2020
(This article belongs to the Collection Cancer Nanomedicine)
Nanotechnology-based approaches hold substantial potential to avoid chemoresistance and minimize side effects. In this work, we have used biocompatible iron oxide magnetic nanoparticles (MNPs) called MF66 and functionalized with the antineoplastic drug doxorubicin (DOX) against MDA-MB-231 cells. Electrostatically functionalized MNPs showed effective uptake and DOX linked to MNPs was more efficiently retained inside the cells than free DOX, leading to cell inactivation by mitotic catastrophe, senescence and apoptosis. Both effects, uptake and cytotoxicity, were demonstrated by different assays and videomicroscopy techniques. Likewise, covalently functionalized MNPs using three different linkers—disulfide (DOX-S-S-Pyr, called MF66-S-S-DOX), imine (DOX-I-Mal, called MF66-I-DOX) or both (DOX-I-S-S-Pyr, called MF66-S-S-I-DOX)—were also analysed. The highest cell death was detected using a linker sensitive to both pH and reducing environment (DOX-I-S-S-Pyr). The greatest success of this study was to detect also their activity against breast cancer stem-like cells (CSC) from MDA-MB-231 and primary breast cancer cells derived from a patient with a similar genetic profile (triple-negative breast cancer). In summary, these nanoformulations are promising tools as therapeutic agent vehicles, due to their ability to produce efficient internalization, drug delivery, and cancer cell inactivation, even in cancer stem-like cells (CSCs) from patients. View Full-Text
Keywords: magnetic iron oxide nanoparticles; doxorubicin; mitotic catastrophe; senescence; apoptosis; cancer stem-like cells magnetic iron oxide nanoparticles; doxorubicin; mitotic catastrophe; senescence; apoptosis; cancer stem-like cells
Show Figures

Graphical abstract

MDPI and ACS Style

Lazaro-Carrillo, A.; Calero, M.; Aires, A.; L. Cortajarena, A.; Simões, B.M.; Latorre, A.; Somoza, Á.; Clarke, R.B.; Miranda, R.; Villanueva, A. Tailored Functionalized Magnetic Nanoparticles to Target Breast Cancer Cells Including Cancer Stem-Like Cells. Cancers 2020, 12, 1397. https://doi.org/10.3390/cancers12061397

AMA Style

Lazaro-Carrillo A, Calero M, Aires A, L. Cortajarena A, Simões BM, Latorre A, Somoza Á, Clarke RB, Miranda R, Villanueva A. Tailored Functionalized Magnetic Nanoparticles to Target Breast Cancer Cells Including Cancer Stem-Like Cells. Cancers. 2020; 12(6):1397. https://doi.org/10.3390/cancers12061397

Chicago/Turabian Style

Lazaro-Carrillo, Ana, Macarena Calero, Antonio Aires, Aitziber L. Cortajarena, Bruno M. Simões, Alfonso Latorre, Álvaro Somoza, Robert B. Clarke, Rodolfo Miranda, and Angeles Villanueva. 2020. "Tailored Functionalized Magnetic Nanoparticles to Target Breast Cancer Cells Including Cancer Stem-Like Cells" Cancers 12, no. 6: 1397. https://doi.org/10.3390/cancers12061397

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop