An Integrated Genomic Strategy to Identify CHRNB4 as a Diagnostic/Prognostic Biomarker for Targeted Therapy in Head and Neck Cancer
Abstract
:1. Introduction
2. Results
2.1. Identification of Smoking-Related and Prognostic Genes in Head and Neck Cancer
2.2. Association Cancer-Related Genes and CHRNB4
2.3. Relationship between Genetic Alterations and CHRNB4 Signature
2.4. The Pathways and Downstream Targets of CHRNB4-High and -Low Subgroups
2.5. Validation of CHRNB4 Gene Editing in HNSCC Cells
2.6. CHRNB4 Promoted Cell Migration and Invasion in NNK-Treated HNSCC Cells
2.7. Varenicline, as a Repurposing Drug of CHRNB4, Inhibited Migration and Invasion in HNSCC Cell Lines
3. Discussion
4. Materials and Methods
4.1. Patient Stratification and RNA-Seq Data Collection
4.2. Methods Overview
4.3. Identification of DEGs
4.4. Survival Analysis
4.5. Cancer Hallmark and Pathway Enrichment Analysis
4.6. Copy Number Alteration (CNA) Analysis
4.7. Cell Lines
4.8. CRISPR/Cas9 Gene Editing on CHRNB4 and Lentivirus Production
4.9. Western Blot Analysis
4.10. Migration and Invasion Assays
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Chuang, Y.-H.; Lee, C.-H.; Lin, C.-Y.; Liu, C.-L.; Huang, S.-H.; Lee, J.-Y.; Chiu, Y.-Y.; Lee, J.-C.; Yang, J.-M. An Integrated Genomic Strategy to Identify CHRNB4 as a Diagnostic/Prognostic Biomarker for Targeted Therapy in Head and Neck Cancer. Cancers 2020, 12, 1324. https://doi.org/10.3390/cancers12051324
Chuang Y-H, Lee C-H, Lin C-Y, Liu C-L, Huang S-H, Lee J-Y, Chiu Y-Y, Lee J-C, Yang J-M. An Integrated Genomic Strategy to Identify CHRNB4 as a Diagnostic/Prognostic Biomarker for Targeted Therapy in Head and Neck Cancer. Cancers. 2020; 12(5):1324. https://doi.org/10.3390/cancers12051324
Chicago/Turabian StyleChuang, Yi-Hsuan, Chia-Hwa Lee, Chun-Yu Lin, Chia-Lin Liu, Sing-Han Huang, Jung-Yu Lee, Yi-Yuan Chiu, Jih-Chin Lee, and Jinn-Moon Yang. 2020. "An Integrated Genomic Strategy to Identify CHRNB4 as a Diagnostic/Prognostic Biomarker for Targeted Therapy in Head and Neck Cancer" Cancers 12, no. 5: 1324. https://doi.org/10.3390/cancers12051324