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Article

Analysis of HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas and Paired Normal Mucosae Reveals Cyclin D1 Deregulation and Compensatory Effect of Cyclin D2

1
Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics of the Czech Academy of Sciences, 142 20 Prague, Czech Republic
2
Department of Informatics and Chemistry, Faculty of Chemical Technology, University of Chemistry and Technology, 160 00 Prague, Czech Republic
3
Institute of Anatomy, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
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Department of Otorhinolaryngology, Head and Neck Surgery, 1st Faculty of Medicine, Charles University, Faculty Hospital Motol, 150 06 Prague, Czech Republic
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Department of Otorhinolaryngology, 3rd Faculty of Medicine, Charles University, 100 00 Prague, Czech Republic
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Institute of Microbiology of the Czech Academy of Sciences, 142 00 Prague, Czech Republic
7
Department of Pathology and Molecular Medicine, Charles University, 2nd Faculty of Medicine and Faculty Hospital Motol, 150 06 Prague, Czech Republic
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Cancers 2020, 12(4), 792; https://doi.org/10.3390/cancers12040792
Received: 24 January 2020 / Revised: 20 March 2020 / Accepted: 21 March 2020 / Published: 26 March 2020
(This article belongs to the Special Issue Human Papillomavirus and Cancers)
Aberrant regulation of the cell cycle is a typical feature of all forms of cancer. In head and neck squamous cell carcinoma (HNSCC), it is often associated with the overexpression of cyclin D1 (CCND1). However, it remains unclear how CCND1 expression changes between tumor and normal tissues and whether human papillomavirus (HPV) affects differential CCND1 expression. Here, we evaluated the expression of D-type cyclins in a cohort of 94 HNSCC patients of which 82 were subjected to whole genome expression profiling of primary tumors and paired normal mucosa. Comparative analysis of paired samples showed that CCND1 was upregulated in 18% of HNSCC tumors. Counterintuitively, CCND1 was downregulated in 23% of carcinomas, more frequently in HPV-positive samples. There was no correlation between the change in D-type cyclin expression and patient survival. Intriguingly, among the tumors with downregulated CCND1, one-third showed an increase in cyclin D2 (CCND2) expression. On the other hand, one-third of tumors with upregulated CCND1 showed a decrease in CCND2. Collectively, we have shown that CCND1 was frequently downregulated in HNSCC tumors. Furthermore, regardless of the HPV status, our data suggested that a change in CCND1 expression was alleviated by a compensatory change in CCND2 expression. View Full-Text
Keywords: human papillomavirus; head and neck squamous cell carcinoma; cell cycle; D-type cyclins; CCND1; CCND2; CCND3; patient survival; paired tumor-normal samples; 11q13 amplification human papillomavirus; head and neck squamous cell carcinoma; cell cycle; D-type cyclins; CCND1; CCND2; CCND3; patient survival; paired tumor-normal samples; 11q13 amplification
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MDPI and ACS Style

Novotný, J.; Bandúrová, V.; Strnad, H.; Chovanec, M.; Hradilová, M.; Šáchová, J.; Šteffl, M.; Grušanović, J.; Kodet, R.; Pačes, V.; Lacina, L.; Smetana, K., Jr.; Plzák, J.; Kolář, M.; Vomastek, T. Analysis of HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas and Paired Normal Mucosae Reveals Cyclin D1 Deregulation and Compensatory Effect of Cyclin D2. Cancers 2020, 12, 792. https://doi.org/10.3390/cancers12040792

AMA Style

Novotný J, Bandúrová V, Strnad H, Chovanec M, Hradilová M, Šáchová J, Šteffl M, Grušanović J, Kodet R, Pačes V, Lacina L, Smetana K Jr., Plzák J, Kolář M, Vomastek T. Analysis of HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas and Paired Normal Mucosae Reveals Cyclin D1 Deregulation and Compensatory Effect of Cyclin D2. Cancers. 2020; 12(4):792. https://doi.org/10.3390/cancers12040792

Chicago/Turabian Style

Novotný, Jiří, Veronika Bandúrová, Hynek Strnad, Martin Chovanec, Miluše Hradilová, Jana Šáchová, Martin Šteffl, Josipa Grušanović, Roman Kodet, Václav Pačes, Lukáš Lacina, Karel Smetana Jr., Jan Plzák, Michal Kolář, and Tomáš Vomastek. 2020. "Analysis of HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas and Paired Normal Mucosae Reveals Cyclin D1 Deregulation and Compensatory Effect of Cyclin D2" Cancers 12, no. 4: 792. https://doi.org/10.3390/cancers12040792

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