β-Caryophyllene Inhibits Cell Proliferation through a Direct Modulation of CB2 Receptors in Glioblastoma Cells
, Giovanni Pallio 1, Alessandra Bitto 1, Federica Mannino 1, Vincenzo Arcoraci 1, Michelangelo Rottura 1, Antonio Ieni 2, Letteria Minutoli 1, Daniela Metro 3, Mario Vaccaro 1, Domenica Altavilla 3 and Francesco Squadrito 1,*
Round 1
Reviewer 1 Report
The authors have addressed satisfactorily the points I raised previously, in light of the response to both reviewers. With the additional data presented and discussion, the paper has substantially improved.
Author Response
Thank you
Reviewer 2 Report
This excellent work explores BCP in two established glioblastoma and one glioblastoma stem cell culture. The authors found that BCP reduced proliferation, cell viability and increased apoptosis. Furthermore, BCP increased pro-apoptosis proteins, decreased anti-apoptotic proteins and furthermore decreased autophagy markers. This data clearly demonstrate that BCP is worth further investigations as possible treatment for glioblastoma. All results are sound and the conclusions drawn are comprehensible. The linguistic style is sufficient for publication. In summary, I strongly support publication.
Further mistakes must be corrected priot to publication:
- The greek letters in the abstract are missing.
- Please change "glioblastoma astrocytoma" in line 22 and line 178 to "glioblastoma".
Author Response
The Reviewer 2 strongly supported the publication of the paper and asked to check and to correct the Greek letters in the abstract and to change “glioblastoma astrocytoma" in line 22 and line 178 to "glioblastoma".
We provided to add the missing Greek letters and we performed the requested changes in lines 22 and 178, using only “glioblastoma”.
This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.
