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Open AccessArticle

New Deployable Expandable Electrodes in the Electroporation Treatment in a Pig Model: A Feasibility and Usability Preliminary Study

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Division of Surgical Oncology, Hepatobiliary Unit, ISTITUTO NAZIONALE TUMORI–IRCCS-FONDAZIONE G. PASCALE, NAPOLI, ITALIA, Via Mariano Semmola, 80131 Naples, Italy
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Division of Surgical Oncology, Maxillo-Facial Unit, ISTITUTO NAZIONALE TUMORI–IRCCS-FONDAZIONE G. PASCALE, NAPOLI, ITALIA, Via Mariano Semmola, 80131 Naples, Italy
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Division of Radiodiagnostic, ISTITUTO NAZIONALE TUMORI–IRCCS-FONDAZIONE G. PASCALE, NAPOLI, ITALIA, Via Mariano Semmola, 80131 Naples, Italy
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Division of General and Oncologic Surgery, Department of Cardiothoracic Sciences, UNIVERSITA’ DEGLI STUDI DELLA CAMPANIA LUIGI VANVITELLI, NAPOLI, ITALIA, 80131 Naples, Italy
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Division of Surgical Oncology, Colo-Rectal Unit, ISTITUTO NAZIONALE TUMORI–IRCCS-FONDAZIONE G. PASCALE, NAPOLI, ITALIA, Via Mariano Semmola, 80131 Naples, Italy
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Research & Development Division, Igea SpA, Via Casarea 65, Casalnuovo di Napoli, 80013 Naples, Italy
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Division of Radiodiagnostic, UNIVERSITA’ DEGLI STUDI DELLA CAMPANIA LUIGI VANVITELLI, NAPOLI, ITALIA, Via Miraglia, 80143 Naples, Italy
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Laboratory Preclinical and Surgical Studies, IRCCS–ISTITUTO ORTOPEDICO RIZZOLI, Via di Barbiano 1/10, 40136 Bologna, Italy
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(2), 515; https://doi.org/10.3390/cancers12020515
Received: 18 January 2020 / Revised: 20 February 2020 / Accepted: 20 February 2020 / Published: 23 February 2020
The aim of the study is to evaluate the usability aspects of new deployable, expandable, electrode prototypes, in terms of suitability solutions for laparoscopic applications on the liver, endoscopic trans-oral and trans-anal procedures, electroporation segmentation in several steps, mechanical functionality (flexibility, penetrability), visibility of the electrode under instrumental guidance, compatibility of the electrode with laparoscopic/endoscopic accesses, surgical instruments, and procedural room and safety compatibility. The electroporation was performed on an animal model (Sus Scrofa Large White 60 kg) both in laparoscopy and endoscopy, under ultrasound guidance, and in open surgery. Electrodes without divergence, with needles coming out straight, parallel to each other, and electrodes with peripheral needles (four needles), diverging from the electrode shaft axis (electrode with non-zero divergence) have been tested. To cause an evaluable necrosis effect, the number of electrical pulses was increased to induce immediate liver cell death. Histological samples were analyzed by staining with Haematoxylin/Eosin or by immunohistochemical staining to confirm complete necrosis. The prototypes of expandable electrodes, tested in laparoscopy and endoscopy and in open surgery, respectively, are suitable in terms of usability, electroporation segmentation in several steps, mechanical functionality (flexibility, penetrability), visibility under instrumental guidance, compatibility with laparoscopic/endoscopic accesses, surgical instruments and procedural room safety, patient safety (no bleeding and/or perforation), and treatment efficacy (adequate ablated volume). Electroporation treatment using new deployable expandable electrode prototypes is safe and feasible. Moreover, electrode configurations allow for a gradual increase in the ablated area in consecutive steps, as confirmed by histology and immunohistochemistry. View Full-Text
Keywords: electroporation; liver; oral cavity; colon-rectal district; new electrodes electroporation; liver; oral cavity; colon-rectal district; new electrodes
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Izzo, F.; Ionna, F.; Granata, V.; Albino, V.; Patrone, R.; Longo, F.; Guida, A.; Delrio, P.; Rega, D.; Scala, D.; Pezzuto, R.; Fusco, R.; Di Bernardo, E.; D’Alessio, V.; Grassi, R.; Contartese, D.; Palaia, R. New Deployable Expandable Electrodes in the Electroporation Treatment in a Pig Model: A Feasibility and Usability Preliminary Study. Cancers 2020, 12, 515.

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