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Article

Trans-Mucosal Efficacy of Non-Thermal Plasma Treatment on Cervical Cancer Tissue and Human Cervix Uteri by a Next Generation Electrosurgical Argon Plasma Device

1
Department of Women’s Health, Eberhard Karls University, 72076 Tübingen, Germany
2
Cluster of Excellence iFIT (EXC 2180) “Image-Guided and Functionally Instructed Tumor Therapies”, Eberhard Karls University, 72076 Tübingen, Germany
3
Natural and Medical Sciences Institute (NMI), 72770 Reutlingen, Germany
4
Department of Medicine/Cardiology, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(2), 267; https://doi.org/10.3390/cancers12020267
Received: 9 December 2019 / Revised: 17 January 2020 / Accepted: 20 January 2020 / Published: 22 January 2020
(This article belongs to the Special Issue Plasma in Cancer Treatment)
Non-invasive physical plasma (NIPP) generated by non-thermally operated electrosurgical argon plasma sources is a promising treatment for local chronic inflammatory, precancerous and cancerous diseases. NIPP-enabling plasma sources are highly available and medically approved. The purpose of this study is the investigation of the effects of non-thermal NIPP on cancer cell proliferation, viability and apoptosis and the identification of the underlying biochemical and molecular modes of action. For this, cervical cancer (CC) single cells and healthy human cervical tissue were analyzed by cell counting, caspase activity assays, microscopic and flow-cytometric viability measurements and molecular tissue characterization using Raman imaging. NIPP treatment caused an immediate and persisting decrease in CC cell growth and cell viability associated with significant plasma-dependent effects on lipid structures. These effects could also be identified in primary cells from healthy cervical tissue and could be traced into the basal cell layer of superficially NIPP-treated cervical mucosa. This study shows that NIPP treatment with non-thermally operated electrosurgical argon plasma devices is a promising method for the treatment of human mucosa, inducing specific molecular changes in cells. View Full-Text
Keywords: tissue penetration; non-thermal plasma; non-invasive plasma treatment (NIPP); cervical intraepithelial neoplasia (CIN); Raman imaging; Raman microspectroscopy; Plasma lipid interactions tissue penetration; non-thermal plasma; non-invasive plasma treatment (NIPP); cervical intraepithelial neoplasia (CIN); Raman imaging; Raman microspectroscopy; Plasma lipid interactions
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MDPI and ACS Style

Wenzel, T.; Carvajal Berrio, D.A.; Reisenauer, C.; Layland, S.; Koch, A.; Wallwiener, D.; Brucker, S.Y.; Schenke-Layland, K.; Brauchle, E.-M.; Weiss, M. Trans-Mucosal Efficacy of Non-Thermal Plasma Treatment on Cervical Cancer Tissue and Human Cervix Uteri by a Next Generation Electrosurgical Argon Plasma Device. Cancers 2020, 12, 267. https://doi.org/10.3390/cancers12020267

AMA Style

Wenzel T, Carvajal Berrio DA, Reisenauer C, Layland S, Koch A, Wallwiener D, Brucker SY, Schenke-Layland K, Brauchle E-M, Weiss M. Trans-Mucosal Efficacy of Non-Thermal Plasma Treatment on Cervical Cancer Tissue and Human Cervix Uteri by a Next Generation Electrosurgical Argon Plasma Device. Cancers. 2020; 12(2):267. https://doi.org/10.3390/cancers12020267

Chicago/Turabian Style

Wenzel, Thomas, Daniel A. Carvajal Berrio, Christl Reisenauer, Shannon Layland, André Koch, Diethelm Wallwiener, Sara Y. Brucker, Katja Schenke-Layland, Eva-Maria Brauchle, and Martin Weiss. 2020. "Trans-Mucosal Efficacy of Non-Thermal Plasma Treatment on Cervical Cancer Tissue and Human Cervix Uteri by a Next Generation Electrosurgical Argon Plasma Device" Cancers 12, no. 2: 267. https://doi.org/10.3390/cancers12020267

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