Next Article in Journal
DUX Hunting—Clinical Features and Diagnostic Challenges Associated with DUX4-Rearranged Leukaemia
Next Article in Special Issue
PUMA and NOXA Expression in Tumor-Associated Benign Prostatic Epithelial Cells Are Predictive of Prostate Cancer Biochemical Recurrence
Previous Article in Journal
Does Fungal Biliary Contamination after Preoperative Biliary Drainage Increase Postoperative Complications after Pancreaticoduodenectomy?
Previous Article in Special Issue
ITPKC as a Prognostic and Predictive Biomarker of Neoadjuvant Chemotherapy for Triple Negative Breast Cancer

Inflammatory Breast Cancer: Clinical Implications of Genomic Alterations and Mutational Profiling

International Research Center, A.C.Camargo Cancer Center, São Paulo 01508-010, Brazil
Department of Clinical Genetics, University Hospital of Southern Denmark, 7100 Vejle, Denmark
Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasília-UnB, Brasília 70910-900, Brazil
Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University-UNESP, Botucatu 18618-681, Brazil
Department of Structural and Functional Biology, Institute of Biosciences, São Paulo State University-UNESP, Botucatu 18618-689, Brazil
Department of Mathematics and Computer Science, University of Southern Denmark, 5230 Odense, Denmark
Department of Breast and Gynecologic Oncology, Barretos Cancer Hospital, Pio XII Foundation, Barretos 14784-390, Brazil
Molecular Oncology Research Center, Barretos SP 14784-400, Brazil
Diagnósticos da América (DASA), Barueri 01525-001, Brazil
Department of Pathology, A.C.Camargo Cancer Center, São Paulo 01525-001, Brazil
TUM School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
Institute of Regional Health Research, University of Southern Denmark, 500 Odense, Denmark
Author to whom correspondence should be addressed.
F.L.C.F. and R.A.R.V. contributed equally to this work.
Cancers 2020, 12(10), 2816;
Received: 28 August 2020 / Revised: 28 September 2020 / Accepted: 29 September 2020 / Published: 30 September 2020
(This article belongs to the Special Issue Novel Biomarkers and Molecular Targets in Cancer)
Inflammatory breast cancer (IBC) is an aggressive disease with high mortality rates. Nowadays, there is no targeted treatment for this tumor type. Based on this context, we investigated the molecular profile of this disease by using well-established methodologies (high-resolution microarray platform, targeted next-generation sequencing, and immunohistochemistry) that have proven potential to unveil cancer biomarkers. We found alterations related to IBC aggressiveness and metastasis (gains of MDM4, losses of CHL1, and high homologous recombination deficiency scores), and worse overall survival (variants in HR and mismatch repair genes). We also compared the mutational profiling of our cases with literature data, which includes both non-IBC and IBC cases, validating our findings. Overall, we describe genetic alterations with the potential to be used as prognostic or predictive biomarkers and ultimately improve IBC patients’ care.
Inflammatory breast cancer (IBC) is a rare and aggressive type of breast cancer whose molecular basis is poorly understood. We performed a comprehensive molecular analysis of 24 IBC biopsies naïve of treatment, using a high-resolution microarray platform and targeted next-generation sequencing (105 cancer-related genes). The genes more frequently affected by gains were MYC (75%) and MDM4 (71%), while frequent losses encompassed TP53 (71%) and RB1 (58%). Increased MYC and MDM4 protein expression levels were detected in 18 cases. These genes have been related to IBC aggressiveness, and MDM4 is a potential therapeutic target in IBC. Functional enrichment analysis revealed genes associated with inflammatory regulation and immune response. High homologous recombination (HR) deficiency scores were detected in triple-negative and metastatic IBC cases. A high telomeric allelic imbalance score was found in patients having worse overall survival (OS). The mutational profiling was compared with non-IBC (TCGA, n = 250) and IBC (n = 118) from four datasets, validating our findings. Higher frequency of TP53 and BRCA2 variants were detected compared to non-IBC, while PIKC3A showed similar frequency. Variants in mismatch repair and HR genes were associated with worse OS. Our study provided a framework for improved diagnosis and therapeutic alternatives for this aggressive tumor type. View Full-Text
Keywords: inflammatory breast cancer; microarray; gene variants; genomic scars; homologous recombination deficiency; copy number alterations inflammatory breast cancer; microarray; gene variants; genomic scars; homologous recombination deficiency; copy number alterations
Show Figures

Figure 1

MDPI and ACS Style

Faldoni, F.L.C.; Villacis, R.A.R.; Canto, L.M.; Fonseca-Alves, C.E.; Cury, S.S.; Larsen, S.J.; Aagaard, M.M.; Souza, C.P.; Scapulatempo-Neto, C.; Osório, C.A.B.T.; Baumbach, J.; Marchi, F.A.; Rogatto, S.R. Inflammatory Breast Cancer: Clinical Implications of Genomic Alterations and Mutational Profiling. Cancers 2020, 12, 2816.

AMA Style

Faldoni FLC, Villacis RAR, Canto LM, Fonseca-Alves CE, Cury SS, Larsen SJ, Aagaard MM, Souza CP, Scapulatempo-Neto C, Osório CABT, Baumbach J, Marchi FA, Rogatto SR. Inflammatory Breast Cancer: Clinical Implications of Genomic Alterations and Mutational Profiling. Cancers. 2020; 12(10):2816.

Chicago/Turabian Style

Faldoni, Flávia L.C., Rolando A.R. Villacis, Luisa M. Canto, Carlos E. Fonseca-Alves, Sarah S. Cury, Simon J. Larsen, Mads M. Aagaard, Cristiano P. Souza, Cristovam Scapulatempo-Neto, Cynthia A.B.T. Osório, Jan Baumbach, Fabio A. Marchi, and Silvia R. Rogatto 2020. "Inflammatory Breast Cancer: Clinical Implications of Genomic Alterations and Mutational Profiling" Cancers 12, no. 10: 2816.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop