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The Autocrine FGF/FGFR System in both Skin and Uveal Melanoma: FGF Trapping as a Possible Therapeutic Approach
Open AccessArticle

The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo Model

1
Department of Ophthalmology, University Hospital Halle (Saale), Martin-Luther University Halle-Wittenberg, Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany
2
Department of Pathology, University Hospital Halle (Saale), Martin-Luther University Halle-Wittenberg, Magdeburger Str. 14, 06112 Halle (Saale), Germany
3
Department of Ophthalmology, Saarland University Medical Center, Kirrberger Str. 100, 66421 Homburg/Saar, Germany
4
Liverpool Ocular Oncology Research Group, Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool, West Derby Street, Liverpool L7 8TX, UK
5
Liverpool Clinical Laboratories, Royal Liverpool University Hospital, Liverpool L69 3GA, UK
*
Author to whom correspondence should be addressed.
Cancers 2019, 11(9), 1344; https://doi.org/10.3390/cancers11091344
Received: 15 August 2019 / Revised: 1 September 2019 / Accepted: 9 September 2019 / Published: 11 September 2019
(This article belongs to the Special Issue Uveal Melanoma)
Uveal melanoma (UM) is the most common primary intraocular tumor that arises from neoplastic melanocytes in the choroid, iris, and ciliary body. Electrochemotherapy (ECT) has been successfully established for the treatment of skin and soft tissue metastatic lesions, deep-seated tumors of the liver, bone metastases, and unresectable pancreas lesions. The aim of this study was to evaluate the effect of ECT in vitro in 3D spheroid culture systems in primary and metastatic UM cell lines. We also investigated the chick embryo chorioallantoic membrane (CAM) as an in vivo model system for the growth and treatment of UM tumors using ECT. The cytotoxic effect of ECT in 3D spheroids was analyzed seven days following treatment by assessment of the size and MTT [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction] assay. The cytotoxicity of ECT after intratumoral or intraarterial administration was evaluated histologically. In vitro and in vivo ECT caused a significant reduction in tumor size and viability compared to electroporation or chemotherapy in both sections of our study. The current results underline the effectiveness of ECT in the treatment of UM and prepare the way for further investigation of its potential application in UM. View Full-Text
Keywords: electrochemotherapy; uveal melanoma; tumor spheroids; chick embryo chorioallantoic membrane; treatment; bleomycin; electroporation; in vitro; in vivo electrochemotherapy; uveal melanoma; tumor spheroids; chick embryo chorioallantoic membrane; treatment; bleomycin; electroporation; in vitro; in vivo
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Fiorentzis, M.; Viestenz, A.; Siebolts, U.; Seitz, B.; Coupland, S.E.; Heinzelmann, J. The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo Model. Cancers 2019, 11, 1344.

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