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Cancers
Volume 11
Issue 3
10.3390/cancers11030380
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Open AccessArticle

Phenotypic Characterization of Circulating Lung Cancer Cells for Clinically Actionable Targets

by
Arutha Kulasinghe
1,2,
Joanna Kapeleris
1,2,
Carolina Cooper
3,4,
Majid Ebrahimi Warkiani
5,6,
Kenneth O’Byrne
2,4 and
Chamindie Punyadeera
1,2,*
1
The School of Biomedical Sciences, Room 603D, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, QLD 4059, Australia
2
Translational Research Institute, Brisbane, QLD 4102, Australia
3
Department of Anatomical Pathology, Pathology Queensland, Brisbane, QLD 4006, Australia
4
Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
5
The School of Biomedical Engineering, University of Technology Sydney, Ultimo, NSW 2007, Australia
6
Department of Biomedical Engineering, Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Moscow 119991, Russia
*
Author to whom correspondence should be addressed.
Cancers 2019, 11(3), 380; https://doi.org/10.3390/cancers11030380
Received: 28 February 2019 / Revised: 14 March 2019 / Accepted: 14 March 2019 / Published: 18 March 2019
(This article belongs to the Special Issue Liquid Biopsy for Cancer)
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Abstract

Objectives: In non-small cell lung cancers (NSCLC), tumour biopsy can often be an invasive procedure. The development of a non-invasive methodology to study genetic changes via circulating tumour cells (CTCs) is an appealing concept. Whilst CTCs typically remain as rare cells, improvements in epitope-independent CTC isolation techniques has given rise to a greater capture of CTCs. In this cross sectional study, we demonstrate the capture and characterization of NSCLC CTCs for the clinically actionable markers epidermal growth factor receptor (EGFR) alterations, anaplastic lymphoma kinase (ALK) rearrangements and programmed death ligand-1 (PD-L1) expression. The study identified CTCs/CTC clusters in 26/35 Stage IV NSCLC patients, and subsequently characterized the CTCs for EGFR mutation, ALK status and PD-L1 status. This pilot study demonstrates the potential of a non-invasive fluid biopsy to determine clinically relevant biomarkers in NSCLC. View Full-Text
Keywords: liquid biopsy; circulating tumour cells; non-small cell lung cancer; actionable mutations liquid biopsy; circulating tumour cells; non-small cell lung cancer; actionable mutations
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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MDPI and ACS Style

Kulasinghe, A.; Kapeleris, J.; Cooper, C.; Warkiani, M.E.; O’Byrne, K.; Punyadeera, C. Phenotypic Characterization of Circulating Lung Cancer Cells for Clinically Actionable Targets. Cancers 2019, 11, 380. https://doi.org/10.3390/cancers11030380

AMA Style

Kulasinghe A, Kapeleris J, Cooper C, Warkiani ME, O’Byrne K, Punyadeera C. Phenotypic Characterization of Circulating Lung Cancer Cells for Clinically Actionable Targets. Cancers. 2019; 11(3):380. https://doi.org/10.3390/cancers11030380

Chicago/Turabian Style

Kulasinghe, Arutha; Kapeleris, Joanna; Cooper, Carolina; Warkiani, Majid E.; O’Byrne, Kenneth; Punyadeera, Chamindie. 2019. "Phenotypic Characterization of Circulating Lung Cancer Cells for Clinically Actionable Targets" Cancers 11, no. 3: 380. https://doi.org/10.3390/cancers11030380

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