Crosstalk between SHH and FGFR Signaling Pathways Controls Tissue Invasion in Medulloblastoma
by
Anuja Neve 1,†, Jessica Migliavacca 1,†, Charles Capdeville 1,†, Marc Thomas Schönholzer 1, Alexandre Gries 1, Min Ma 2, Karthiga Santhana Kumar 1, Michael Grotzer 1 and Martin Baumgartner 1,*
Anuja Neve 1,†, Jessica Migliavacca 1,†, Charles Capdeville 1,†, Marc Thomas Schönholzer 1, Alexandre Gries 1, Min Ma 2, Karthiga Santhana Kumar 1, Michael Grotzer 1 and Martin Baumgartner 1,*
1
Department of Oncology, University Children’s Hospital Zürich, CH-8032 Zürich, Switzerland
2
Faculty of Biology and Medicine, University of Lausanne, Biochemistry, CH-1066 Epalinges, Switzerland
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Cancers 2019, 11(12), 1985; https://doi.org/10.3390/cancers11121985
Received: 31 October 2019 / Revised: 25 November 2019 / Accepted: 6 December 2019 / Published: 10 December 2019
(This article belongs to the Special Issue Pediatric Brain Tumor)
In the Sonic Hedgehog (SHH) subgroup of medulloblastoma (MB), tumor initiation and progression are in part driven by smoothened (SMO) and fibroblast growth factor (FGF)-receptor (FGFR) signaling, respectively. We investigated the impact of the SMO-FGFR crosstalk on tumor growth and invasiveness in MB. We found that FGFR signaling represses GLI1 expression downstream of activated SMO in the SHH MB line DAOY and induces MKI67, HES1, and BMI1 in DAOY and in the group 3 MB line HD-MBO3. FGFR repression of GLI1 does not affect proliferation or viability, whereas inhibition of FGFR is necessary to release SMO-driven invasiveness. Conversely, SMO activation represses FGFR-driven sustained activation of nuclear ERK. Parallel activation of FGFR and SMO in ex vivo tumor cell-cerebellum slice co-cultures reduced invasion of tumor cells without affecting proliferation. In contrast, treatment of the cells with the SMO antagonist Sonidegib (LDE225) blocked invasion and proliferation in cerebellar slices. Thus, sustained, low-level SMO activation is necessary for proliferation and tissue invasion, whereas acute, pronounced activation of SMO can repress FGFR-driven invasiveness. This suggests that the tumor cell response is dependent on the relative local abundance of the two factors and indicates a paradigm of microenvironmental control of invasion in SHH MB through mutual control of SHH and FGFR signaling.
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Keywords:
medulloblastoma; FGFR; Sonic Hedgehog signaling; organotypic culture; cell invasion; signal crosstalk; MAP kinase signaling
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MDPI and ACS Style
Neve, A.; Migliavacca, J.; Capdeville, C.; Schönholzer, M.T.; Gries, A.; Ma, M.; Santhana Kumar, K.; Grotzer, M.; Baumgartner, M. Crosstalk between SHH and FGFR Signaling Pathways Controls Tissue Invasion in Medulloblastoma. Cancers 2019, 11, 1985.
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