The Actin Binding Protein Plastin-3 Is Involved in the Pathogenesis of Acute Myeloid Leukemia
Abstract
:1. Introduction
2. Results
2.1. The Actin Binding Protein PLS3 Is Expressed in Primary AML Cells
2.2. Functional Analysis of PSL3 by Knockdown or Overexpression in Kasumi-1 Cells
2.3. Knockdown of PLS3 Significantly Improves Survival In Vivo
2.4. PLS3 Represents a Negative Prognostic Marker in AML
3. Discussion
4. Materials and Methods
4.1. Patients and Samples
4.2. Cell Culture
4.3. Reverse Transcription Quantitative PCR Analysis
4.4. Immunofluorescence Staining
4.5. Lentiviral Transduction of Kasumi-1 Cells with PLS3-Specific shRNA or a PLS3 Overexpression Construct
4.6. Staining of F-Actin
4.7. Proliferation Assays
4.8. Colony Formation Assay
4.9. Xenograft Model
4.10. RNA Sequencing
4.11. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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FAB Subtype | PLS3 (%) | ||
---|---|---|---|
Low | High | Fisher’s Exact Test | |
M1 (n = 67) | 81 | 19 | p = 0.077 |
M2 (n = 64) | 94 | 6 | |
M4 (n = 59) | 93 | 7 | |
M5 (n = 62) | 97 | 13 |
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Velthaus, A.; Cornils, K.; Hennigs, J.K.; Grüb, S.; Stamm, H.; Wicklein, D.; Bokemeyer, C.; Heuser, M.; Windhorst, S.; Fiedler, W.; et al. The Actin Binding Protein Plastin-3 Is Involved in the Pathogenesis of Acute Myeloid Leukemia. Cancers 2019, 11, 1663. https://doi.org/10.3390/cancers11111663
Velthaus A, Cornils K, Hennigs JK, Grüb S, Stamm H, Wicklein D, Bokemeyer C, Heuser M, Windhorst S, Fiedler W, et al. The Actin Binding Protein Plastin-3 Is Involved in the Pathogenesis of Acute Myeloid Leukemia. Cancers. 2019; 11(11):1663. https://doi.org/10.3390/cancers11111663
Chicago/Turabian StyleVelthaus, Arne, Kerstin Cornils, Jan K. Hennigs, Saskia Grüb, Hauke Stamm, Daniel Wicklein, Carsten Bokemeyer, Michael Heuser, Sabine Windhorst, Walter Fiedler, and et al. 2019. "The Actin Binding Protein Plastin-3 Is Involved in the Pathogenesis of Acute Myeloid Leukemia" Cancers 11, no. 11: 1663. https://doi.org/10.3390/cancers11111663