Identification of SERPINE1 as a Regulator of Glioblastoma Cell Dispersal with Transcriptome Profiling
Abstract
:1. Introduction
2. Results
2.1. Transcriptome Profiling of Motile and Non-Motile GBM Cells Reveal Major Alterations in Cell Proliferation and Movement Pathways
2.2. SERPINE1 Inhibition Reduces GBM Dispersal
2.3. SERPINE1 Knock-Down Reduces Cell Adhesion and Directional Persistence of GBM Cells
2.4. TGFβ Is an Upstream Regulator of SERPINE1
2.5. SERPINE1 Expression Is Associated with Poor Patient Survival and Its Silencing in A Clinically-Relevant Model Reduces Dispersal
2.6. SERPINE1 Knock-Down Reduces Tumor Progression In Vivo
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Reagents
4.2. Generation of Tumor Cell Spheroids
4.3. Dispersal Assays
4.4. Dispersal Area Analysis
4.5. Wound Healing Assays
4.6. RNA Sequencing (RNA-seq) and Transcriptome Profiling of Core and Migratory Cells
4.7. qRT-PCR Experiments
4.8. Cloning and Packaging of Silencing Vectors
4.9. Western Blotting
4.10. Cell Viability Experiments
4.11. Immunofluorescence Staining
4.12. Adhesion Experiments
4.13. Single-Cell Tracking and Persistence Analysis
4.14. Patient Survival Analysis
4.15. Live Cell Imaging Experiments
4.16. In Vivo Experiments
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Seker, F.; Cingoz, A.; Sur-Erdem, İ.; Erguder, N.; Erkent, A.; Uyulur, F.; Esai Selvan, M.; Gümüş, Z.H.; Gönen, M.; Bayraktar, H.; et al. Identification of SERPINE1 as a Regulator of Glioblastoma Cell Dispersal with Transcriptome Profiling. Cancers 2019, 11, 1651. https://doi.org/10.3390/cancers11111651
Seker F, Cingoz A, Sur-Erdem İ, Erguder N, Erkent A, Uyulur F, Esai Selvan M, Gümüş ZH, Gönen M, Bayraktar H, et al. Identification of SERPINE1 as a Regulator of Glioblastoma Cell Dispersal with Transcriptome Profiling. Cancers. 2019; 11(11):1651. https://doi.org/10.3390/cancers11111651
Chicago/Turabian StyleSeker, Fidan, Ahmet Cingoz, İlknur Sur-Erdem, Nazli Erguder, Alp Erkent, Fırat Uyulur, Myvizhi Esai Selvan, Zeynep Hülya Gümüş, Mehmet Gönen, Halil Bayraktar, and et al. 2019. "Identification of SERPINE1 as a Regulator of Glioblastoma Cell Dispersal with Transcriptome Profiling" Cancers 11, no. 11: 1651. https://doi.org/10.3390/cancers11111651