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Exploiting NK Cell Surveillance Pathways for Cancer Therapy

Functional Assessment for Clinical Use of Serum-Free Adapted NK-92 Cells

Center for Hematology and Regenerative Medicine, Department of Medicine Huddinge, Karolinska Institutet, 141 83 Stockholm, Sweden
NSU Cell Therapy Institute, Nova Southeastern University, Fort Lauderdale, FL 33314, USA
Haematology Centre, Karolinska University Hospital, 141 57 Huddinge, Sweden
Nanotechnology Research and Application Center, Sabanci University, 34956 Istanbul, Turkey
Author to whom correspondence should be addressed.
Authors contributed equally.
Cancers 2019, 11(1), 69;
Received: 15 November 2018 / Revised: 28 December 2018 / Accepted: 3 January 2019 / Published: 10 January 2019
(This article belongs to the Special Issue Natural Killer Cells and Cancer Therapy)
Natural killer (NK) cells stand out as promising candidates for cellular immunotherapy due to their capacity to kill malignant cells. However, the therapeutic use of NK cells is often dependent on cell expansion and activation with considerable amounts of serum and exogenous cytokines. We aimed to develop an expansion protocol for NK-92 cells in an effort to generate a cost-efficient, xeno-free, clinical grade manufactured master cell line for therapeutic applications. By making functional assays with NK-92 cells cultured under serum-free conditions (NK-92SF) and comparing to serum-supplemented NK-92 cells (NK-92S) we did not observe significant alterations in the viability, proliferation, receptor expression levels, or in perforin and granzyme levels. Interestingly, even though NK-92SF cells displayed decreased degranulation and cytotoxicity against tumor cells in vitro, the degranulation capacity was recovered after overnight incubation with 20% serum in the medium. Moreover, lentiviral vector-based genetic modification efficiency of NK-92SF cells was comparable with NK-92S cells. The application of similar strategies can be useful in reducing the costs of manufacturing cells for clinical use and can help us understand and implement strategies towards chemically defined expansion and genetic modification protocols. View Full-Text
Keywords: NK-92; NK cell; serum-free; immunotherapy NK-92; NK cell; serum-free; immunotherapy
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MDPI and ACS Style

Chrobok, M.; Dahlberg, C.I.M.; Sayitoglu, E.C.; Beljanski, V.; Nahi, H.; Gilljam, M.; Stellan, B.; Sutlu, T.; Duru, A.D.; Alici, E. Functional Assessment for Clinical Use of Serum-Free Adapted NK-92 Cells. Cancers 2019, 11, 69.

AMA Style

Chrobok M, Dahlberg CIM, Sayitoglu EC, Beljanski V, Nahi H, Gilljam M, Stellan B, Sutlu T, Duru AD, Alici E. Functional Assessment for Clinical Use of Serum-Free Adapted NK-92 Cells. Cancers. 2019; 11(1):69.

Chicago/Turabian Style

Chrobok, Michael, Carin I.M. Dahlberg, Ece C. Sayitoglu, Vladimir Beljanski, Hareth Nahi, Mari Gilljam, Birgitta Stellan, Tolga Sutlu, Adil D. Duru, and Evren Alici. 2019. "Functional Assessment for Clinical Use of Serum-Free Adapted NK-92 Cells" Cancers 11, no. 1: 69.

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