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Open AccessFeature PaperCommunication
Micromachines 2018, 9(5), 235; https://doi.org/10.3390/mi9050235

Assembly and Connection of Micropatterned Single Neurons for Neuronal Network Formation

1
Center for International Research on Integrative Biomedical Systems, Institute of Industrial Science, The University of Tokyo, 4-6-1 Meguro-ku, Komaba, Tokyo 153-8505, Japan
2
International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo Institutes for Advanced Study (UTIAS), The University of Tokyo, Tokyo 153-8505, Japan
*
Author to whom correspondence should be addressed.
Received: 6 April 2018 / Revised: 4 May 2018 / Accepted: 7 May 2018 / Published: 15 May 2018
(This article belongs to the Special Issue State-of-the-Art Lab-on-a-Chip Technology in Japan)
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Abstract

Engineering of neuronal network geometry by micropatterning technology is a key future technology for creating artificial brains on a chip. However, engineering of network geometry at the single-cell-level with functional morphology (axon/dendrite) and connectivity (synapses) is still challenging. Here, we describe a method for controlling the axon and dendrite morphology of single primary-cultured neurons and assembling a neural circuit using mobile microplates. The microplates enabled morphological control of neurons by their shapes and bringing their ends into contact caused the formation of physical connections. Functional synapse formation at the connection was indicated by immunostaining of synapse-related proteins and intracellular Ca2+ imaging of neural activity. We believe that the method will be useful in engineering neural circuits with selected neurons and defined morphology. View Full-Text
Keywords: neuronal network; micropatterning; micromanipulation; hippocampal neuron; synapse neuronal network; micropatterning; micromanipulation; hippocampal neuron; synapse
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Yoshida, S.; Kato-Negishi, M.; Takeuchi, S. Assembly and Connection of Micropatterned Single Neurons for Neuronal Network Formation. Micromachines 2018, 9, 235.

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