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Micromachines 2018, 9(4), 140; https://doi.org/10.3390/mi9040140

A Microfluidic Lab-on-a-Disc (LOD) for Antioxidant Activities of Plant Extracts

1
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia
2
Centre for Innovation in Medical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia
3
School of Engineering and Sciences, Tecnologico de Monterrey, Av. Eugenio Garza Sada 2501 Sur, 64849 Monterrey, NL, Mexico
4
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
5
Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA
*
Author to whom correspondence should be addressed.
Received: 13 January 2018 / Revised: 15 March 2018 / Accepted: 19 March 2018 / Published: 21 March 2018
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Abstract

Antioxidants are an important substance that can fight the deterioration of free radicals and can easily oxidize when exposed to light. There are many methods to measure the antioxidant activity in a biological sample, for example 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity test, which is one of the simplest methods used. Despite its simplicity, the organic solvent that has been used to dilute DPPH is easily evaporated and degraded with respect to light exposure and time. Thus, it needs to be used at the earliest convenient time prior to the experiment. To overcome this issue, a rapid and close system for antioxidant activity is required. In this paper, we introduced the Lab-on-a-Disc (LoD) method that integrates the DPPH antioxidant activity test on a microfluidic compact disc (CD). We used ascorbic acid, quercetin, Areca catechu, Polygonum minus, and Syzygium polyanthum plant extracts to compare the results of our proposed LoD method with the conventional method. Contrasted to the arduous laborious conventional method, our proposed method offer rapid analysis and simple determination of antioxidant. This proposed LoD method for antioxidant activity in plants would be a platform for the further development of antioxidant assay. View Full-Text
Keywords: Lab-on-a-Disc (LoD); centrifugal microfluidic CD; plant antioxidant activity; antioxidants; DPPH Lab-on-a-Disc (LoD); centrifugal microfluidic CD; plant antioxidant activity; antioxidants; DPPH
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Abd Rahman, N.; Ibrahim, F.; Aeinehvand, M.M.; Yusof, R.; Madou, M. A Microfluidic Lab-on-a-Disc (LOD) for Antioxidant Activities of Plant Extracts. Micromachines 2018, 9, 140.

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