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Self-Renewal and Differentiation of Adipose-Derived Stem Cells (ADSCs) Stimulated by Multi-Axial Tensile Strain in a Pneumatic Microdevice

1
Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan
2
Bone and Joint Research Center, Chang Gung Memorial Hospital, Linkou 333, Taiwan
3
Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Linkou 333, Taiwan
4
Graduate Institute of Biomedical Engineering, Chang Gung University, Taoyuan 333, Taiwan
5
Department of Radiation Oncology, Chang Gung Memorial Hospital, Linkou 333, Taiwan
*
Authors to whom correspondence should be addressed.
Micromachines 2018, 9(11), 607; https://doi.org/10.3390/mi9110607
Received: 21 September 2018 / Revised: 15 November 2018 / Accepted: 15 November 2018 / Published: 19 November 2018
(This article belongs to the Section B:Biology)
Adipose-derived stem cells (ADSCs) were suggested for treating degenerative osteoarthritis, suppressing inflammatory responses, and repairing damaged soft tissues. Moreover, the ADSCs have the potential to undergo self-renewal and differentiate into bone, tendon, cartilage, and ligament. Recently, investigation of the self-renewal and differentiation of the ADSCs has become an attractive area. In this work, a pneumatic microdevice has been developed to study the gene expression of the ADSCs after the stimulation of multi-axial tensile strain. The ADSCs were cultured on the microdevice and experienced multi-axial tensile strain during a three-day culture course. Self-renewal and differentiation abilities were investigated by mRNA expressions of NANOG, sex determining region Y-box 2 (SOX2), octamer-binding transcription factor 4 (OCT4), sex determining region Y-box9 (SOX9), peroxisome proliferator-activated receptor gamma (PPAR-γ), and runt-related transcription factor 2 (RUNX2). The result showed that the genes related self-renewal were significantly up-regulated after the tensile stimulation. Higher proliferation ratio of the ADSCs was also shown by cell viability assay. The microdevice provides a promising platform for cell-based study under mechanical tensile stimulation. View Full-Text
Keywords: microdevice; tensile stimulation; adipose-derived stem cells; self-renewal; differentiation microdevice; tensile stimulation; adipose-derived stem cells; self-renewal; differentiation
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MDPI and ACS Style

Chiu, C.-H.; Tong, Y.-W.; Yeh, W.-L.; Lei, K.F.; Chen, A. .-Y. Self-Renewal and Differentiation of Adipose-Derived Stem Cells (ADSCs) Stimulated by Multi-Axial Tensile Strain in a Pneumatic Microdevice. Micromachines 2018, 9, 607.

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