Next Article in Journal
Large-Scale Fabrication of Porous Gold Nanowires via Laser Interference Lithography and Dealloying of Gold–Silver Nano-Alloys
Next Article in Special Issue
Analysis of the Diffusion Process by pH Indicator in Microfluidic Chips for Liposome Production
Previous Article in Journal
Microtechnologies for Cell Microenvironment Control and Monitoring
Previous Article in Special Issue
Preparation of Protein Nanoparticles Using NTA End Functionalized Polystyrenes on the Interface of a Multi-Laminated Flow Formed in a Microchannel
Open AccessFeature PaperArticle

Investigation of Drug Cocktail Effects on Cancer Cell-Spheroids Using a Microfluidic Drug-Screening Assay

Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China
City University of Hong Kong Shenzhen Research Institute, Shenzhen 518057, China
Centre for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Hong Kong, China
Author to whom correspondence should be addressed.
The authors contributed equally to this work.
Academic Editors: Xunli Zhang and Dario Carugo
Micromachines 2017, 8(6), 167;
Received: 18 April 2017 / Revised: 18 May 2017 / Accepted: 19 May 2017 / Published: 24 May 2017
(This article belongs to the Special Issue Microfluidic Technologies for Drug Delivery)
Development of drugs based on potential anti-cancer chemotherapeutic agents has been hindered by its necessary tedious procedures and failure in the clinical trials because of unbearable toxicity and extremely low clinical efficacy. One of the technical challenges is the mismatch between laboratory settings and human body environments for the cancer cells responding upon treatments of the anti-cancer agents. This major limitation urges for applying more reliable platforms for evaluating drugs with a higher throughput and cell aggregates in a more natural configuration. Here, we adopt a microfluidic device integrated with a differential micromixer and multiple microwell-containing channels (50 microwells per channel) for parallel screening of suspending cell spheroids treated by drugs with different combinations. We optimize the culture conditions of the surfactant-coated microwells in order to facilitate the spheroid formation of the breast cancer cell line (MDA-MB-231). We propose a new drug cocktail combined with three known chemotherapeutic agents (paclitaxel, epirubicin, and aspirin) for the drug screening of the cancer cell-spheroids. Our results exhibit the differential responses between planar cell layers in traditional culture wells and cell-spheroids grown in our microfluidic device, in terms of the apoptotic rates under treatments of the drug cocktails with different concentrations. These results reveal a distinct drug resistance between planar cell layers and cell-spheroids. Together, this work offers important guidelines on applying the cell-spheroid microfluidic cultures for development of more efficacious anticancer drugs. View Full-Text
Keywords: microfluidic; toxicity; cell; cancer; drug screening microfluidic; toxicity; cell; cancer; drug screening
Show Figures

Figure 1

MDPI and ACS Style

Au Ieong, K.I.; Yang, C.; Wong, C.T.; Shui, A.C.; Wu, T.T.Y.; Chen, T.-H.; Lam, R.H.W. Investigation of Drug Cocktail Effects on Cancer Cell-Spheroids Using a Microfluidic Drug-Screening Assay. Micromachines 2017, 8, 167.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop