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Micromachines 2017, 8(3), 83;

A Review on Macroscale and Microscale Cell Lysis Methods

Department of Mechanical Engineering, McMaster University, Hamilton, ON L8S 4L7, Canada
Author to whom correspondence should be addressed.
Academic Editors: Aaron T. Ohta and Wenqi Hu
Received: 21 January 2017 / Revised: 26 February 2017 / Accepted: 3 March 2017 / Published: 8 March 2017
(This article belongs to the Special Issue Microdevices and Microsystems for Cell Manipulation)
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The lysis of cells in order to extract the nucleic acids or proteins inside it is a crucial unit operation in biomolecular analysis. This paper presents a critical evaluation of the various methods that are available both in the macro and micro scale for cell lysis. Various types of cells, the structure of their membranes are discussed initially. Then, various methods that are currently used to lyse cells in the macroscale are discussed and compared. Subsequently, popular methods for micro scale cell lysis and different microfluidic devices used are detailed with their advantages and disadvantages. Finally, a comparison of different techniques used in microfluidics platform has been presented which will be helpful to select method for a particular application. View Full-Text
Keywords: cell lysis; cell lysis methods; microfluidics; electrical lysis; mechanical lysis; thermal lysis cell lysis; cell lysis methods; microfluidics; electrical lysis; mechanical lysis; thermal lysis

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Shehadul Islam, M.; Aryasomayajula, A.; Selvaganapathy, P.R. A Review on Macroscale and Microscale Cell Lysis Methods. Micromachines 2017, 8, 83.

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