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Micromachines 2016, 7(8), 140;

A Method of Three-Dimensional Micro-Rotational Flow Generation for Biological Applications

Department of Mechanical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
Laboratory for Integrated Biodevice, Quantitative Biology Center, RIKEN, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan
Division of Pharmacology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya, Tokyo 158-8501, Japan
Global Center for Advanced Medical Engineering and Informatics, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
Authors to whom correspondence should be addressed.
Academic Editors: Manabu Tokeshi and Kiichi Sato
Received: 29 April 2016 / Revised: 28 June 2016 / Accepted: 29 July 2016 / Published: 10 August 2016
(This article belongs to the Special Issue Micro/Nano Devices for Chemical Analysis)
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We report a convenient method to create a three-dimensional micro-rotational fluidic platform for biological applications in the direction of a vertical plane (out-of-plane) without contact in an open space. Unlike our previous complex fluidic manipulation system, this method uses a micro-rotational flow generated near a single orifice when the solution is pushed from the orifice by using a single pump. The three-dimensional fluidic platform shows good potential for fluidic biological applications such as culturing, stimulating, sorting, and manipulating cells. The pattern and velocity of the micro-rotational flow can be controlled by tuning the parameters such as the flow rate and the liquid-air interface height. We found that bio-objects captured by the micro-rotational flow showed self-rotational motion and orbital motion. Furthermore, the path length and position, velocity, and pattern of the orbital motion of the bio-object could be controlled. To demonstrate our method, we used embryoid body cells. As a result, the orbital motion had a maximum length of 2.4 mm, a maximum acceleration of 0.63 m/s2, a frequency of approximately 0.45 Hz, a maximum velocity of 15.4 mm/s, and a maximum rotation speed of 600 rpm. The capability to have bio-objects rotate or move orbitally in three dimensions without contact opens up new research opportunities in three-dimensional microfluidic technology. View Full-Text
Keywords: three-dimensional microfluidic platform; micro-rotational flow; non-contact; open space three-dimensional microfluidic platform; micro-rotational flow; non-contact; open space

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Yalikun, Y.; Kanda, Y.; Morishima, K. A Method of Three-Dimensional Micro-Rotational Flow Generation for Biological Applications. Micromachines 2016, 7, 140.

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