Combining Electro-Osmotic Flow and FTA® Paper for DNA Analysis on Microfluidic Devices
Abstract
:1. Introduction
2. Materials and Methods
2.1. Manufacture and Functionalisation of Microfluidic Devices
2.2. Sample Preparation
2.3. DNA Purification Procedure
2.3.1. Conventional Method
2.3.2. Microfluidic Method
2.4. DNA Quantification
2.5. DNA Amplification Procedure
3. Results and Discussion
3.1. Optimisation of Electro-Osmotic Movement
3.2. Integrated DNA Purification and Amplification
3.3. Analysis of Different Sample Types
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
DNA | Deoxyribonucleic acid |
DTT | Dithiothreitol |
EDTA | Ethylenediaminetetraacetic acid |
EOF | Electro-osmotic flow |
PCR | Polymerase chain reaction |
qPCR | Real-time quantitative PCR |
TE | Tris/EDTA buffer |
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Applied Voltage (Vcm−1) | 50 | 75 | 100 | 125 | 150 |
---|---|---|---|---|---|
FTA® paper | 77.8% | 83.8% | 87.3% | 42.9% | 29.8% |
Anode | - | - | - | 17.3% | 26.8% |
Cathode | - | - | - | - | - |
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Wimbles, R.; Melling, L.M.; Shaw, K.J. Combining Electro-Osmotic Flow and FTA® Paper for DNA Analysis on Microfluidic Devices. Micromachines 2016, 7, 119. https://doi.org/10.3390/mi7070119
Wimbles R, Melling LM, Shaw KJ. Combining Electro-Osmotic Flow and FTA® Paper for DNA Analysis on Microfluidic Devices. Micromachines. 2016; 7(7):119. https://doi.org/10.3390/mi7070119
Chicago/Turabian StyleWimbles, Ryan, Louise M. Melling, and Kirsty J. Shaw. 2016. "Combining Electro-Osmotic Flow and FTA® Paper for DNA Analysis on Microfluidic Devices" Micromachines 7, no. 7: 119. https://doi.org/10.3390/mi7070119