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Magnetophoretic Sorting of Single Cell-Containing Microdroplets

Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
Samsung Electronics, 4 Seocho-daero 74-gil, Seocho-gu, Seoul 06620, Republic of Korea
Author to whom correspondence should be addressed.
Academic Editor: Abel Martin Gonzalez Oliva
Micromachines 2016, 7(4), 56;
Received: 27 February 2016 / Revised: 23 March 2016 / Accepted: 28 March 2016 / Published: 30 March 2016
(This article belongs to the Special Issue Advances in Microfluidic Devices for Cell Handling and Analysis)
Droplet microfluidics is a promising tool for single-cell analysis since single cell can be comparted inside a tiny volume. However, droplet encapsulation of single cells still remains a challenging issue due to the low ratio of droplets containing single cells. Here, we introduce a simple and robust single cell sorting platform based on a magnetophoretic method using monodisperse magnetic nanoparticles (MNPs) and droplet microfluidics with >94% purity. There is an approximately equal amount of MNPs in the same-sized droplet, which has the same magnetic force under the magnetic field. However, the droplets containing single cells have a reduced number of MNPs, as much as the volume of the cell inside the droplet, resulting in a low magnetic force. Based on this simple principle, this platform enables the separation of single cell-encapsulated droplets from the droplets with no cells. Additionally, this device uses only a permanent magnet without any complex additional apparatus; hence, this new platform can be integrated into a single cell analysis system considering its effectiveness and convenience. View Full-Text
Keywords: magnetic nanoparticles; magnetophoresis; separation; single cell; Thalassiosira eccentrica magnetic nanoparticles; magnetophoresis; separation; single cell; Thalassiosira eccentrica
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MDPI and ACS Style

Jo, Y.; Shen, F.; Hahn, Y.K.; Park, J.-H.; Park, J.-K. Magnetophoretic Sorting of Single Cell-Containing Microdroplets. Micromachines 2016, 7, 56.

AMA Style

Jo Y, Shen F, Hahn YK, Park J-H, Park J-K. Magnetophoretic Sorting of Single Cell-Containing Microdroplets. Micromachines. 2016; 7(4):56.

Chicago/Turabian Style

Jo, Younggeun, Fengshan Shen, Young K. Hahn, Ji-Ho Park, and Je-Kyun Park. 2016. "Magnetophoretic Sorting of Single Cell-Containing Microdroplets" Micromachines 7, no. 4: 56.

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