Next Article in Journal
Resonant Varifocal Micromirror with Piezoresistive Focus Sensor
Next Article in Special Issue
Application of Vertical Electrodes in Microfluidic Channels for Impedance Analysis
Previous Article in Journal
An Analytical Model for CMUTs with Square Multilayer Membranes Using the Ritz Method
Previous Article in Special Issue
Particulate Blood Analogues Reproducing the Erythrocytes Cell-Free Layer in a Microfluidic Device Containing a Hyperbolic Contraction
Article

Magnetophoretic Sorting of Single Cell-Containing Microdroplets

1
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
2
Samsung Electronics, 4 Seocho-daero 74-gil, Seocho-gu, Seoul 06620, Republic of Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Abel Martin Gonzalez Oliva
Micromachines 2016, 7(4), 56; https://doi.org/10.3390/mi7040056
Received: 27 February 2016 / Revised: 23 March 2016 / Accepted: 28 March 2016 / Published: 30 March 2016
(This article belongs to the Special Issue Advances in Microfluidic Devices for Cell Handling and Analysis)
Droplet microfluidics is a promising tool for single-cell analysis since single cell can be comparted inside a tiny volume. However, droplet encapsulation of single cells still remains a challenging issue due to the low ratio of droplets containing single cells. Here, we introduce a simple and robust single cell sorting platform based on a magnetophoretic method using monodisperse magnetic nanoparticles (MNPs) and droplet microfluidics with >94% purity. There is an approximately equal amount of MNPs in the same-sized droplet, which has the same magnetic force under the magnetic field. However, the droplets containing single cells have a reduced number of MNPs, as much as the volume of the cell inside the droplet, resulting in a low magnetic force. Based on this simple principle, this platform enables the separation of single cell-encapsulated droplets from the droplets with no cells. Additionally, this device uses only a permanent magnet without any complex additional apparatus; hence, this new platform can be integrated into a single cell analysis system considering its effectiveness and convenience. View Full-Text
Keywords: magnetic nanoparticles; magnetophoresis; separation; single cell; Thalassiosira eccentrica magnetic nanoparticles; magnetophoresis; separation; single cell; Thalassiosira eccentrica
Show Figures

Graphical abstract

MDPI and ACS Style

Jo, Y.; Shen, F.; Hahn, Y.K.; Park, J.-H.; Park, J.-K. Magnetophoretic Sorting of Single Cell-Containing Microdroplets. Micromachines 2016, 7, 56. https://doi.org/10.3390/mi7040056

AMA Style

Jo Y, Shen F, Hahn YK, Park J-H, Park J-K. Magnetophoretic Sorting of Single Cell-Containing Microdroplets. Micromachines. 2016; 7(4):56. https://doi.org/10.3390/mi7040056

Chicago/Turabian Style

Jo, Younggeun, Fengshan Shen, Young K. Hahn, Ji-Ho Park, and Je-Kyun Park. 2016. "Magnetophoretic Sorting of Single Cell-Containing Microdroplets" Micromachines 7, no. 4: 56. https://doi.org/10.3390/mi7040056

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop