Next Article in Journal
Design Optimization and Fabrication of High-Sensitivity SOI Pressure Sensors with High Signal-to-Noise Ratios Based on Silicon Nanowire Piezoresistors
Next Article in Special Issue
Microfluidic Platform for Cell Isolation and Manipulation Based on Cell Properties
Previous Article in Journal
Review of Microfluidic Photobioreactor Technology for Metabolic Engineering and Synthetic Biology of Cyanobacteria and Microalgae
Previous Article in Special Issue
Fabrication of a Cell Fixation Device for Robotic Cell Microinjection
Article Menu

Export Article

Open AccessArticle
Micromachines 2016, 7(10), 186;

Cell Migration According to Shape of Graphene Oxide Micropatterns

Department of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Korea
Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, Korea
Research Center for Energy Convergence Technology, Pusan National University, Busan 46241, Korea
Dental Life Science Research Institute, Seoul National University Dental Hospital, Seoul 03080, Korea
Department of Mechanical Engineering, Sejong University, Seoul 05006, Korea
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Academic Editors: Chang-Hwan Choi, Aaron T. Ohta and Wenqi Hu
Received: 26 August 2016 / Revised: 5 October 2016 / Accepted: 7 October 2016 / Published: 14 October 2016
(This article belongs to the Special Issue Microdevices and Microsystems for Cell Manipulation)
Full-Text   |   PDF [3551 KB, uploaded 14 October 2016]   |  


Photolithography is a unique process that can effectively manufacture micro/nano-sized patterns on various substrates. On the other hand, the meniscus-dragging deposition (MDD) process can produce a uniform surface of the substrate. Graphene oxide (GO) is the oxidized form of graphene that has high hydrophilicity and protein absorption. It is widely used in biomedical fields such as drug delivery, regenerative medicine, and tissue engineering. Herein, we fabricated uniform GO micropatterns via MDD and photolithography. The physicochemical properties of the GO micropatterns were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), and Raman spectroscopy. Furthermore, cell migration on the GO micropatterns was investigated, and the difference in cell migration on triangle and square GO micropatterns was examined for their effects on cell migration. Our results demonstrated that the GO micropatterns with a desired shape can be finely fabricated via MDD and photolithography. Moreover, it was revealed that the shape of GO micropatterns plays a crucial role in cell migration distance, speed, and directionality. Therefore, our findings suggest that the GO micropatterns can serve as a promising biofunctional platform and cell-guiding substrate for applications to bioelectric devices, cell-on-a-chip, and tissue engineering scaffolds. View Full-Text
Keywords: photolithography; meniscus-dragging deposition; graphene oxide; micropatterns; cell migration photolithography; meniscus-dragging deposition; graphene oxide; micropatterns; cell migration

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary materials

  • Supplementary File 1:

    Supplementary (ZIP, 4814 KB)

  • Externally hosted supplementary file 1
    Doi: none
    Link: http://none
    Description: Video S1: Time-lapse video of L929 fibroblasts on triangle GO micropatterns for 12 hours. Video S2: Time-lapse video of L929 fibroblasts on square GO micropatterns for 12 hours.
Printed Edition Available!
A printed edition of this Special Issue is available here.

Share & Cite This Article

MDPI and ACS Style

Kim, S.E.; Kim, M.S.; Shin, Y.C.; Eom, S.U.; Lee, J.H.; Shin, D.-M.; Hong, S.W.; Kim, B.; Park, J.-C.; Shin, B.S.; Lim, D.; Han, D.-W. Cell Migration According to Shape of Graphene Oxide Micropatterns. Micromachines 2016, 7, 186.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top