Next Article in Journal
Rotational Efficiency of Photo-Driven Archimedes Screws for Micropumps
Next Article in Special Issue
Effect of Laser-Induced Heating on Raman Measurement within a Silicon Microfluidic Channel
Previous Article in Journal / Special Issue
On-Chip Transportation and Measurement of Mechanical Characteristics of Oocytes in an Open Environment
Article Menu

Export Article

Open AccessArticle
Micromachines 2015, 6(6), 660-673;

On-Chip Method to Measure Mechanical Characteristics of a Single Cell by Using Moiré Fringe

Department of Mechanical Engineering, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya 464-8603, Japan
Department of Mechanical Engineering, Osaka University, Yamadaoka 2-1, Suita 565-0871, Japan
Author to whom correspondence should be addressed.
Academic Editor: Jeong-Bong Lee
Received: 23 April 2015 / Revised: 23 May 2015 / Accepted: 29 May 2015 / Published: 3 June 2015
(This article belongs to the Collection Lab-on-a-Chip)
Full-Text   |   PDF [8441 KB, uploaded 3 June 2015]   |  


We propose a method to characterize the mechanical properties of cells using a robot-integrated microfluidic chip (robochip) and microscopy. The microfluidic chip is designed to apply the specified deformations to a single detached cell using an on-chip actuator probe. The reaction force is simultaneously measured using an on-chip force sensor composed of a hollow folded beam and probe structure. In order to measure the cellular characteristics in further detail, a sub-pixel level of resolution of probe position is required. Therefore, we utilize the phase detection of moiré fringe. Using this method, the experimental resolution of the probe position reaches 42 nm. This is approximately ten times smaller than the optical wavelength, which is the limit of sharp imaging with a microscope. Calibration of the force sensor is also important in accurately measuring cellular reaction forces. We calibrated the spring constant from the frequency response, by the proposed sensing method of the probe position. As a representative of mechanical characteristics, we measured the elastic modulus of Madin-Darby Cannie Kidney (MDCK) cells. In spite of the rigid spring constant, the resolution and sensitivity were twice that achieved in our previous study. Unique cellular characteristics can be elucidated by the improvements in sensing resolution and accuracy. View Full-Text
Keywords: moiré fringe; lab-on-a-chip; cellular measurement; force sensing moiré fringe; lab-on-a-chip; cellular measurement; force sensing

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Sugiura, H.; Sakuma, S.; Kaneko, M.; Arai, F. On-Chip Method to Measure Mechanical Characteristics of a Single Cell by Using Moiré Fringe. Micromachines 2015, 6, 660-673.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top