Bronchoalveolar Lavage Fluid from Chronic Obstructive Pulmonary Disease Patients Increases Neutrophil Chemotaxis Measured by a Microfluidic Platform
Abstract
:1. Introduction
2. Methods
2.1. Bronchoalveolar Lavage Fluid (BALF) Sample Collection and Preparation
2.2. Neutrophil Preparation
2.3. Microfluidic Device Preparation
2.4. Microfluidic Chemotaxis Experiment
2.5. Drug Response Experiment
2.6. Data Analysis
3. Results
3.1. Neutrophil Chemotaxis in COPD BALF, Control BALF, and Known Recombinant Chemoattractants
3.2. Effect of Dexamethasone on Neutrophil Chemotaxis
3.3. Linear Regression Analyses of Neutrophil migration in COPD BALF against Patients’ Clinical Data
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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No. | Gender | Age | FEV1 (L) | FEV1 (%) | FVC (L) | FVC (%) | FEV1/FVC (%) | |
---|---|---|---|---|---|---|---|---|
COPD | 1 | M | 61 | 0.82 | 29.60 | 2.18 | 60.30 | 37.63 |
2 | M | 72 | 1.53 | 52.41 | 3.37 | 87.59 | 45.35 | |
3 | M | 59 | 1.76 | 54.92 | 2.85 | 70.45 | 61.74 | |
4 | M | 71 | 0.89 | 35.11 | 2.08 | 60.87 | 43.01 | |
5 | M | 68 | 0.79 | 40.51 | 1.75 | 70.23 | 45.32 | |
6 | M | 65 | 1.38 | 45.80 | 2.78 | 71.70 | 49.80 | |
7 | M | 67 | 1.55 | 59.07 | 2.87 | 85.13 | 54.01 | |
8 | M | 63 | 1.98 | 69.00 | 2.99 | 79.19 | 66.00 | |
Control | 1 | F | 53 | 2.13 | 91.80 | 2.8 | 102.20 | 76.16 |
2 | M | 46 | 3.37 | 97.78 | 4.69 | 110.52 | 71.88 | |
3 | M | 40 | 3.72 | 97.63 | 4.5 | 97.37 | 82.80 | |
4 | M | 46 | 3.74 | 116.49 | 4.46 | 114.40 | 83.84 | |
5 | M | 36 | 3.69 | 99.14 | 4.45 | 99.79 | 82.96 | |
6 | F | 57 | 2.48 | 111.48 | 2.88 | 105.65 | 86.14 | |
7 | M | 38 | 3.65 | 95.65 | 4.49 | 97.87 | 81.16 |
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Ren, J.; Chen, W.; Zhong, Z.; Wang, N.; Chen, X.; Yang, H.; Li, J.; Tang, P.; Fan, Y.; Lin, F.; et al. Bronchoalveolar Lavage Fluid from Chronic Obstructive Pulmonary Disease Patients Increases Neutrophil Chemotaxis Measured by a Microfluidic Platform. Micromachines 2023, 14, 1740. https://doi.org/10.3390/mi14091740
Ren J, Chen W, Zhong Z, Wang N, Chen X, Yang H, Li J, Tang P, Fan Y, Lin F, et al. Bronchoalveolar Lavage Fluid from Chronic Obstructive Pulmonary Disease Patients Increases Neutrophil Chemotaxis Measured by a Microfluidic Platform. Micromachines. 2023; 14(9):1740. https://doi.org/10.3390/mi14091740
Chicago/Turabian StyleRen, Jiaqi, Wenfang Chen, Zhicheng Zhong, Ning Wang, Xi Chen, Hui Yang, Jing Li, Ping Tang, Yanping Fan, Francis Lin, and et al. 2023. "Bronchoalveolar Lavage Fluid from Chronic Obstructive Pulmonary Disease Patients Increases Neutrophil Chemotaxis Measured by a Microfluidic Platform" Micromachines 14, no. 9: 1740. https://doi.org/10.3390/mi14091740