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Article

An Improved Transwell Design for Microelectrode Ion-Flux Measurements

1
School of Medical Engineering and Applied Social Sciences, University of Applied Sciences Upper Austria, Garnisonstr. 21, 4020 Linz, Austria
2
Institute of Microbiology of the Czech Academy of Sciences, Zamek 136, 37333 Nove Hrady, Czech Republic
3
Institute of Applied Physics, Johannes Kepler University Linz, Altenberger Str. 69, 4040 Linz, Austria
*
Author to whom correspondence should be addressed.
Authors contributed equally.
Academic Editor: András Dér
Micromachines 2021, 12(3), 273; https://doi.org/10.3390/mi12030273
Received: 5 February 2021 / Revised: 1 March 2021 / Accepted: 3 March 2021 / Published: 6 March 2021
(This article belongs to the Special Issue Versatile Organ-on-a-Chip Devices)
The microelectrode ion flux estimation (MIFE) is a powerful, non-invasive electrophysiological method for cellular membrane transport studies. Usually, the MIFE measurements are performed in a tissue culture dish or directly with tissues (roots, parts of the plants, and cell tissues). Here, we present a transwell system that allows for MIFE measurements on a cell monolayer. We introduce a measurement window in the transwell insert membrane, which provides direct access for the cells to the media in the upper and lower compartment of the transwell system and allows direct cell-to-cell contact coculture. Three-dimensional multiphoton lithography (MPL) was used to construct a 3D grid structure for cell support in the measurement window. The optimal polymer grid constant was found for implementation in transwell MIFE measurements. We showed that human umbilical vein endothelial cells (HUVECs) efficiently grow and maintain their physiological response on top of the polymer structures. View Full-Text
Keywords: multiphoton lithography; biocompatible polymers; BisSR; HUVECs; MIFE; ion flux multiphoton lithography; biocompatible polymers; BisSR; HUVECs; MIFE; ion flux
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MDPI and ACS Style

Buchroithner, B.; Spurný, P.; Mayr, S.; Heitz, J.; Sivun, D.; Jacak, J.; Ludwig, J. An Improved Transwell Design for Microelectrode Ion-Flux Measurements. Micromachines 2021, 12, 273. https://doi.org/10.3390/mi12030273

AMA Style

Buchroithner B, Spurný P, Mayr S, Heitz J, Sivun D, Jacak J, Ludwig J. An Improved Transwell Design for Microelectrode Ion-Flux Measurements. Micromachines. 2021; 12(3):273. https://doi.org/10.3390/mi12030273

Chicago/Turabian Style

Buchroithner, Boris, Pavel Spurný, Sandra Mayr, Johannes Heitz, Dmitry Sivun, Jaroslaw Jacak, and Jost Ludwig. 2021. "An Improved Transwell Design for Microelectrode Ion-Flux Measurements" Micromachines 12, no. 3: 273. https://doi.org/10.3390/mi12030273

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