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Article

A Pillar-Free Diffusion Device for Studying Chemotaxis on Supported Lipid Bilayers

by 1, 1, 1 and 1,2,3,*
1
Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA
2
Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033, USA
3
USC Stem Cell, University of Southern California, Los Angeles, CA 90033, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Khashayar Khoshmanesh and Sara Baratchi
Micromachines 2021, 12(10), 1254; https://doi.org/10.3390/mi12101254
Received: 22 September 2021 / Revised: 15 October 2021 / Accepted: 15 October 2021 / Published: 16 October 2021
(This article belongs to the Special Issue Microfluidics Technologies for Cell-Based Assays, Volume II)
Chemotactic cell migration plays a crucial role in physiological and pathophysiological processes. In tissues, cells can migrate not only through extracellular matrix (ECM), but also along stromal cell surfaces via membrane-bound receptor–ligand interactions to fulfill critical functions. However, there remains a lack of models recapitulating chemotactic migration mediated through membrane-bound interactions. Here, using micro-milling, we engineered a multichannel diffusion device that incorporates a chemoattractant gradient and a supported lipid bilayer (SLB) tethered with membrane-bound factors that mimics stromal cell membranes. The chemoattractant channels are separated by hydrogel barriers from SLB in the cell loading channel, which enable precise control of timing and profile of the chemokine gradients applied on cells interacting with SLB. The hydrogel barriers are formed in pillar-free channels through a liquid pinning process, which eliminates complex cleanroom-based fabrications and distortion of chemoattractant gradient by pillars in typical microfluidic hydrogel barrier designs. As a proof-of-concept, we formed an SLB tethered with ICAM-1, and demonstrated its lateral mobility and different migratory behavior of Jurkat T cells on it from those on immobilized ICAM-1, under a gradient of chemokine CXCL12. Our platform can thus be widely used to investigate membrane-bound chemotaxis such as in cancer, immune, and stem cells. View Full-Text
Keywords: chemotaxis; micro-milling; microdevice; supported lipid bilayer; membrane-bound interactions; ICAM-1; CXCL12 chemotaxis; micro-milling; microdevice; supported lipid bilayer; membrane-bound interactions; ICAM-1; CXCL12
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MDPI and ACS Style

Hao, J.; Zhao, W.; Oh, J.M.; Shen, K. A Pillar-Free Diffusion Device for Studying Chemotaxis on Supported Lipid Bilayers. Micromachines 2021, 12, 1254. https://doi.org/10.3390/mi12101254

AMA Style

Hao J, Zhao W, Oh JM, Shen K. A Pillar-Free Diffusion Device for Studying Chemotaxis on Supported Lipid Bilayers. Micromachines. 2021; 12(10):1254. https://doi.org/10.3390/mi12101254

Chicago/Turabian Style

Hao, Jia, Winfield Zhao, Jeong M. Oh, and Keyue Shen. 2021. "A Pillar-Free Diffusion Device for Studying Chemotaxis on Supported Lipid Bilayers" Micromachines 12, no. 10: 1254. https://doi.org/10.3390/mi12101254

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