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A Portable Device for Simple Exosome Separation from Biological Samples

by 1,2,†, 1,2,†, 3, 3, 1,2, 1,2, 1,2,4,* and 3,*
1
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
2
University of Chinese Academy of Sciences, Beijing 100864, China
3
First Affiliated Hospital of Dalian Medical University, Dalian 116011, China
4
CAS Centre for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China
*
Authors to whom correspondence should be addressed.
These authors equally contributed to this work.
Academic Editor: Aiqun Liu
Micromachines 2021, 12(10), 1182; https://doi.org/10.3390/mi12101182
Received: 2 September 2021 / Revised: 24 September 2021 / Accepted: 28 September 2021 / Published: 29 September 2021
Exosomes are membrane-bound nanovesicles secreted by most types of cells, which contain a series of biologically important molecules, such as miRNAs, proteins, and lipids, etc. Emerging evidence show that exosomes can affect the physiological status of cells and are involved in various pathological processes. However, due to their small size and density close to body fluids, it is challenging to separate exosomes from a small volume of biological samples in a simple manner. Herein, we propose a new strategy for isolating circulating exosomes from biological samples in a portable device. This method synergistically integrates chitosan electrostatic-adsorption, scaffold substrates, and shuttle flow to enable the highly effective capture of circulating exosomes with a recovery rate of over 80% within 20 min, which is much better than the performance of traditional ultracentrifugation (5–25%, 3 h). Besides, the isolated exosomes from samples could be lysed in situ and further subjected to RNA concentration detection and protein analysis. In particular, all the necessary procedures for exosome separation could be integrated into a single device without the need for bulky equipment. This established device is portable and easy to operate, which provides a promising platform for the study of exosome biology and clinical diagnosis. View Full-Text
Keywords: exosome; separation; chitosan scaffold exosome; separation; chitosan scaffold
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MDPI and ACS Style

Chen, W.; Xie, Y.; Chang, Y.; Xu, Y.; Zhao, M.; Deng, P.; Qin, J.; Li, H. A Portable Device for Simple Exosome Separation from Biological Samples. Micromachines 2021, 12, 1182. https://doi.org/10.3390/mi12101182

AMA Style

Chen W, Xie Y, Chang Y, Xu Y, Zhao M, Deng P, Qin J, Li H. A Portable Device for Simple Exosome Separation from Biological Samples. Micromachines. 2021; 12(10):1182. https://doi.org/10.3390/mi12101182

Chicago/Turabian Style

Chen, Wenwen, Yingying Xie, Yuang Chang, Yuhai Xu, Mengqian Zhao, Pengwei Deng, Jianhua Qin, and Hongjing Li. 2021. "A Portable Device for Simple Exosome Separation from Biological Samples" Micromachines 12, no. 10: 1182. https://doi.org/10.3390/mi12101182

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