Next Article in Journal
Randomly Multiplexed Diffractive Lens and Axicon for Spatial and Spectral Imaging
Next Article in Special Issue
Shape Memory Alloy Capsule Micropump for Drug Delivery Applications
Previous Article in Journal
Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene
Article

A 3D Printed Self-Sustainable Cell-Encapsulation Drug Delivery Device for Periocular Transplant-Based Treatment of Retinal Degenerative Diseases

1
Department of Finemechanics, Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki, Aoba-ku, Sendai 980-8579, Japan
2
Division of Clinical Cell Therapy, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, 2-1 Seiryo, Aoba-ku, Sendai 980-8575, Japan
3
Department of Biomedical Engineering, Graduate School of Biomedical Engineering, Tohoku University, 6-6-01 Aramaki, Aoba-ku, Sendai 980-8579, Japan
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(4), 436; https://doi.org/10.3390/mi11040436
Received: 27 March 2020 / Revised: 15 April 2020 / Accepted: 18 April 2020 / Published: 21 April 2020
(This article belongs to the Special Issue Advanced Drug Delivery Devices)
Self-sustainable release of brain-derived neurotrophic factor (BDNF) to the retina using minimally invasive cell-encapsulation devices is a promising approach to treat retinal degenerative diseases (RDD). Herein, we describe such a self-sustainable drug delivery device with human retinal pigment epithelial (ARPE-19) cells (cultured on collagen coated polystyrene (PS) sheets) enclosed inside a 3D printed semi-porous capsule. The capsule was 3D printed with two photo curable polymers: triethylene glycol dimethacrylate (TEGDM) and polyethylene glycol dimethylacrylate (PEGDM). The capsule’s semi-porous membrane (PEGDM) could serve three functions: protecting the cells from body’s immune system by limiting diffusion (5.97 ± 0.11%) of large molecules like immunoglobin G (IgG)(150 kDa); helping the cells to survive inside the capsule by allowing diffusion (43.20 ± 2.16%) of small molecules (40 kDa) like oxygen and necessary nutrients; and helping in the treatment of RDD by allowing diffusion of cell-secreted BDNF to the outside environment. In vitro results showed a continuous BDNF secretion from the device for at least 16 days, demonstrating future potential of the cell-encapsulation device for the treatment of RDD in a minimally invasive and self-sustainable way through a periocular transplant. View Full-Text
Keywords: retinal degenerative disease; cell-encapsulation device; periocular implant; growth factors; brain-derived neurotrophic factor (BDNF); cell sheet engineering; 3D printing; minimally invasive device retinal degenerative disease; cell-encapsulation device; periocular implant; growth factors; brain-derived neurotrophic factor (BDNF); cell sheet engineering; 3D printing; minimally invasive device
Show Figures

Figure 1

MDPI and ACS Style

Kojima, H.; Raut, B.; Chen, L.-J.; Nagai, N.; Abe, T.; Kaji, H. A 3D Printed Self-Sustainable Cell-Encapsulation Drug Delivery Device for Periocular Transplant-Based Treatment of Retinal Degenerative Diseases. Micromachines 2020, 11, 436. https://doi.org/10.3390/mi11040436

AMA Style

Kojima H, Raut B, Chen L-J, Nagai N, Abe T, Kaji H. A 3D Printed Self-Sustainable Cell-Encapsulation Drug Delivery Device for Periocular Transplant-Based Treatment of Retinal Degenerative Diseases. Micromachines. 2020; 11(4):436. https://doi.org/10.3390/mi11040436

Chicago/Turabian Style

Kojima, Hideto, Bibek Raut, Li-Jiun Chen, Nobuhiro Nagai, Toshiaki Abe, and Hirokazu Kaji. 2020. "A 3D Printed Self-Sustainable Cell-Encapsulation Drug Delivery Device for Periocular Transplant-Based Treatment of Retinal Degenerative Diseases" Micromachines 11, no. 4: 436. https://doi.org/10.3390/mi11040436

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop