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Open AccessArticle

Cell Lysis Based on an Oscillating Microbubble Array

by Xiufang Liu 1,2,†, Jinyuan Li 1,2,†, Liangyu Zhang 1, Xiaowei Huang 2, Umar Farooq 2, Na Pang 1,2, Wei Zhou 2, Lin Qi 1, Lisheng Xu 1,*, Lili Niu 2,3,* and Long Meng 2,3,*
1
College of Medicine and Biological Information Engineering, Northeastern University, 195 Innovation Road, Shenyang 110016, China
2
Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Avenue, Shenzhen 518055, China
3
Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Guangzhou 510515, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Micromachines 2020, 11(3), 288; https://doi.org/10.3390/mi11030288
Received: 14 December 2019 / Revised: 4 March 2020 / Accepted: 8 March 2020 / Published: 10 March 2020
(This article belongs to the Special Issue Optimization of Microfluidic Devices)
Cell lysis is a process of breaking cell membranes to release intracellular substances such as DNA, RNA, protein, or organelles from a cell. The detection of DNA, RNA, or protein from the lysed cells is of importance for cancer diagnostics and drug screening. In this study, we develop a microbubble array that enables the realization of multiple cell lysis induced by the shear stress resulting from the individual oscillating microbubbles. The oscillating microbubbles in the channel have similar vibration amplitudes, and the intracellular substances can be released from the individual cells efficiently. Moreover, the efficiency of cell lysis increases with increments of input voltage and sonication time. By means of DNA agarose-gel electrophoresis, a sufficient extraction amount of DNA released from the lysed cells can be detected, and there is no significant difference in lysis efficiency when compared to cell lysis achieved using commercial kits. With the advantages of the simple manufacturing process, low cost, high efficiency, and high speed, this device can serve as an efficient and versatile tool for the single-cell sequencing of cell biology research, disease diagnosis, and stem cell therapy. View Full-Text
Keywords: cell lysis; ultrasound bioeffects; stable cavitation; shear stress cell lysis; ultrasound bioeffects; stable cavitation; shear stress
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MDPI and ACS Style

Liu, X.; Li, J.; Zhang, L.; Huang, X.; Farooq, U.; Pang, N.; Zhou, W.; Qi, L.; Xu, L.; Niu, L.; Meng, L. Cell Lysis Based on an Oscillating Microbubble Array. Micromachines 2020, 11, 288.

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