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Open AccessArticle

Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes

1
Multiscale Manufacturing Laboratory, Mechanical Engineering Department, Clemson University, Clemson, SC 29634, USA
2
Institute for Microstructure Technology, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
3
Department of Bioengineering, 301 Rhodes Engineering Research Center, Clemson University, Clemson, SC 29634, USA
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(3), 255; https://doi.org/10.3390/mi11030255
Received: 3 February 2020 / Revised: 23 February 2020 / Accepted: 26 February 2020 / Published: 28 February 2020
(This article belongs to the Special Issue Micro/Nano-Chip Electrokinetics, Volume III)
Bloodstream infection with Candida fungal cells remains one of the most life-threatening complications among hospitalized patients around the world. Although most of the cases are still due to Candida albicans, the rising incidence of infections caused by other Candida strains that may not respond to traditional anti-fungal treatments merits the development of a method for species-specific isolation of Candida. To this end, here we present the characterization of the dielectrophoresis (DEP) response of Candida albicans, Candida tropicalis and Candida parapsilosis. We complement such characterization with a study of the Candida cells morphology. The Candida strains exhibited subtle differences in their morphology and dimensions. All the Candida strains exhibited positive DEP in the range 10–500 kHz, although the strength of the DEP response was different for each Candida strain at different frequencies. Only Candida tropicalis showed positive DEP at 750 kHz. The current results show potential for manipulation and enrichment of a specific Candida strain at specific DEP conditions towards aiding in the rapid identification of Candida strains to enable the effective and timely treatment of Candida infections. View Full-Text
Keywords: characterization; dielectrophoresis; carbon electrodes; three-dimensional (3D); diagnostics; Candidiasis characterization; dielectrophoresis; carbon electrodes; three-dimensional (3D); diagnostics; Candidiasis
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Islam, M.; Keck, D.; Gilmore, J.; Martinez-Duarte, R. Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes. Micromachines 2020, 11, 255.

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