Next Article in Journal
Phospholipase D from Loxosceles laeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
Next Article in Special Issue
Baseline Susceptibility of Field Populations of Helicoverpa armigera to Bacillus thuringiensis Vip3Aa Toxin and Lack of Cross-Resistance between Vip3Aa and Cry Toxins
Previous Article in Journal / Special Issue
Use of Carabids for the Post-Market Environmental Monitoring of Genetically Modified Crops
Open AccessArticle

Patterns of Gene Expression in Western Corn Rootworm (Diabrotica virgifera virgifera) Neonates, Challenged with Cry34Ab1, Cry35Ab1 and Cry34/35Ab1, Based on Next-Generation Sequencing

1
Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583-0915, USA
2
Center for Biotechnology, School of Biological Sciences, UNL, Lincoln, NE 68583, USA
3
Dow AgroSciences, Indianapolis, IN 46268, USA
4
Monsanto, St. Louis, MO 63167, USA
5
Entomology and Nematology Department, University of Florida, Gainesville, FL 32611-0620, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Juan Ferré and Baltasar Escriche
Toxins 2017, 9(4), 124; https://doi.org/10.3390/toxins9040124
Received: 26 February 2017 / Revised: 22 March 2017 / Accepted: 27 March 2017 / Published: 30 March 2017
(This article belongs to the Special Issue The Insecticidal Bacterial Toxins in Modern Agriculture)
With Next Generation Sequencing technologies, high-throughput RNA sequencing (RNAseq) was conducted to examine gene expression in neonates of Diabrotica virgifera virgifera (LeConte) (Western Corn Rootworm, WCR) challenged with individual proteins of the binary Bacillus thuringiensis insecticidal proteins, Cry34Ab1 and Cry35Ab1, and the combination of Cry34/Cry35Ab1, which together are active against rootworm larvae. Integrated results of three different statistical comparisons identified 114 and 1300 differentially expressed transcripts (DETs) in the Cry34Ab1 and Cry34/35Ab1 treatment, respectively, as compared to the control. No DETs were identified in the Cry35Ab1 treatment. Putative Bt binding receptors previously identified in other insect species were not identified in DETs in this study. The majority of DETs (75% with Cry34Ab1 and 68.3% with Cry34/35Ab1 treatments) had no significant hits in the NCBI nr database. In addition, 92 DETs were shared between Cry34Ab1 and Cry34/35Ab1 treatments. Further analysis revealed that the most abundant DETs in both Cry34Ab1 and Cry34/35Ab1 treatments were associated with binding and catalytic activity. Results from this study confirmed the nature of these binary toxins against WCR larvae and provide a fundamental profile of expression pattern of genes in response to challenge of the Cry34/35Ab1 toxin, which may provide insight into potential resistance mechanisms. View Full-Text
Keywords: Next Generation Sequencing (NGS); Diabrotica virgifera virgifera; Bt challenge; differential gene expression Next Generation Sequencing (NGS); Diabrotica virgifera virgifera; Bt challenge; differential gene expression
Show Figures

Figure 1

MDPI and ACS Style

Wang, H.; Eyun, S.-i.; Arora, K.; Tan, S.Y.; Gandra, P.; Moriyama, E.; Khajuria, C.; Jurzenski, J.; Li, H.; Donahue, M.; Narva, K.; Siegfried, B. Patterns of Gene Expression in Western Corn Rootworm (Diabrotica virgifera virgifera) Neonates, Challenged with Cry34Ab1, Cry35Ab1 and Cry34/35Ab1, Based on Next-Generation Sequencing. Toxins 2017, 9, 124. https://doi.org/10.3390/toxins9040124

AMA Style

Wang H, Eyun S-i, Arora K, Tan SY, Gandra P, Moriyama E, Khajuria C, Jurzenski J, Li H, Donahue M, Narva K, Siegfried B. Patterns of Gene Expression in Western Corn Rootworm (Diabrotica virgifera virgifera) Neonates, Challenged with Cry34Ab1, Cry35Ab1 and Cry34/35Ab1, Based on Next-Generation Sequencing. Toxins. 2017; 9(4):124. https://doi.org/10.3390/toxins9040124

Chicago/Turabian Style

Wang, Haichuan; Eyun, Seong-il; Arora, Kanika; Tan, Sek Y.; Gandra, Premchand; Moriyama, Etsuko; Khajuria, Chitvan; Jurzenski, Jessica; Li, Huarong; Donahue, Maia; Narva, Ken; Siegfried, Blair. 2017. "Patterns of Gene Expression in Western Corn Rootworm (Diabrotica virgifera virgifera) Neonates, Challenged with Cry34Ab1, Cry35Ab1 and Cry34/35Ab1, Based on Next-Generation Sequencing" Toxins 9, no. 4: 124. https://doi.org/10.3390/toxins9040124

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop