Silver Nanoparticle-Based Fluorescence-Quenching Lateral Flow Immunoassay for Sensitive Detection of Ochratoxin A in Grape Juice and Wine
Abstract
:1. Introduction
2. Results and Discussion
2.1. Scheme of AgNP–RuNP–cLFIA Sensor
2.2. Characterization of AgNPs, RuNPs, and AgNP Probes
2.3. Parameter Optimization of AgNP–RuNP–cLFIA Sensor
2.4. Performance Evaluation of AgNP–RuNP–cLFIA Sensor
3. Conclusions
4. Materials and Methods
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample | Spiking Levels (μg/L) | Dilution Factors | Mean Value (μg/L) * | SD % | CV % | Recovery % |
---|---|---|---|---|---|---|
Grape wine | 0.40 | 4 | 0.11 | 0.94 | 8.54 | 110.0 |
2.00 | 4 | 0.44 | 3.01 | 6.84 | 88.0 | |
5.00 | 4 | 1.26 | 2.28 | 1.81 | 100.8 | |
Grape juice | 0.40 | 2 | 0.22 | 0.76 | 3.45 | 110.0 |
2.00 | 2 | 0.92 | 3.59 | 3.90 | 92.0 | |
5.00 | 2 | 2.56 | 4.80 | 1.87 | 102.4 |
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Jiang, H.; Li, X.; Xiong, Y.; Pei, K.; Nie, L.; Xiong, Y. Silver Nanoparticle-Based Fluorescence-Quenching Lateral Flow Immunoassay for Sensitive Detection of Ochratoxin A in Grape Juice and Wine. Toxins 2017, 9, 83. https://doi.org/10.3390/toxins9030083
Jiang H, Li X, Xiong Y, Pei K, Nie L, Xiong Y. Silver Nanoparticle-Based Fluorescence-Quenching Lateral Flow Immunoassay for Sensitive Detection of Ochratoxin A in Grape Juice and Wine. Toxins. 2017; 9(3):83. https://doi.org/10.3390/toxins9030083
Chicago/Turabian StyleJiang, Hu, Xiangmin Li, Ying Xiong, Ke Pei, Lijuan Nie, and Yonghua Xiong. 2017. "Silver Nanoparticle-Based Fluorescence-Quenching Lateral Flow Immunoassay for Sensitive Detection of Ochratoxin A in Grape Juice and Wine" Toxins 9, no. 3: 83. https://doi.org/10.3390/toxins9030083