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Open AccessArticle

Rapid Assessment of the Toxicity of Fungal Compounds Using Luminescent Vibrio qinghaiensis sp. Q67

by Qijie Jian 1,2,†, Liang Gong 1,†, Taotao Li 1,2, Yong Wang 3, Yu Wu 1,2, Feng Chen 4, Hongxia Qu 1, Xuewu Duan 1 and Yueming Jiang 1,*
1
Key Laboratory of Plant Resource Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
2
School of Life Sciences, University of Chinese Academy of Sciences, Beijing 100039, China
3
Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528403, China
4
Department of Food, Nutrition and Packaging Sciences, Clemson University, Clemson, South Carolina, SC 29634, USA
*
Author to whom correspondence should be addressed.
These two authors contribute equally to this work.
Academic Editor: Massimo Reverberi
Toxins 2017, 9(10), 335; https://doi.org/10.3390/toxins9100335
Received: 2 October 2017 / Revised: 16 October 2017 / Accepted: 17 October 2017 / Published: 21 October 2017
(This article belongs to the Section Mycotoxins)
Most tropical fruits after harvest are very perishable because of fungal infection. Since some pathogenic fungi can produce hazardous compounds such as mycotoxins, novel rapid and effective methods to assess those hazardous compounds are urgently needed. Herein we report that Vibrio qinghaiensis sp. Q67, a luminescent bacterium, can be used to rapidly assess the toxicities of mycotoxins and cultures from mycotoxin-producing pathogens. A good correlation (R2 > 0.98) between concentrations of the mycotoxins (fumonisin B1, deoxynivalenol, zearalenone, ochratoxin A, patulin, and citrinin) and the luminous intensity of V. qinghaiensis sp. Q67 was obtained. Furthermore, significant correlations (R2 > 0.96) between the amount of mycotoxin and the luminous intensity from the cultures of 10 major mycotoxin-producing pathogens were also observed. In addition, Fusarium proliferatum (half-maximal inhibitory concentration (IC50) = 17.49%) exhibited greater luminescence suppression than Fusarium semitectum (IC50 = 92.56%) or Fusarium oxysporum (IC50 = 28.61%), which was in agreement with the existing higher levels of fumonisin B1, fumonisin B2, and deoxynivalenol, which were measured by high-performance liquid chromatography-tandem mass spectrometry. These results suggest that V. qinghaiensis sp. Q67 is a promising alternative for the rapid evaluation of the toxicity of fungal mycotoxins. View Full-Text
Keywords: toxicity; mycotoxin; bioluminescence; Vibrio qinghaiensis sp. Q67 toxicity; mycotoxin; bioluminescence; Vibrio qinghaiensis sp. Q67
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MDPI and ACS Style

Jian, Q.; Gong, L.; Li, T.; Wang, Y.; Wu, Y.; Chen, F.; Qu, H.; Duan, X.; Jiang, Y. Rapid Assessment of the Toxicity of Fungal Compounds Using Luminescent Vibrio qinghaiensis sp. Q67. Toxins 2017, 9, 335.

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