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Toxins 2017, 9(10), 317;

Response of Intestinal Bacterial Flora to the Long-term Feeding of Aflatoxin B1 (AFB1) in Mice

Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China
Author to whom correspondence should be addressed.
Academic Editors: Isabelle P. Oswald, Philippe Pinton and Imourana Alassane-Kpembi
Received: 9 August 2017 / Revised: 28 September 2017 / Accepted: 30 September 2017 / Published: 12 October 2017
(This article belongs to the Special Issue Effects of Mycotoxins on the Intestine)
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In order to investigate the influence of aflatoxin B1 (AFB1) on intestinal bacterial flora, 24 Kunming mice (KM mice) were randomly placed into four groups, which were labeled as control, low-dose, medium-dose, and high-dose groups. They were fed intragastrically with 0.4 mL of 0 mg/L, 2.5 mg/L, 4 mg/L, or 10 mg/L of AFB1 solutions, twice a day for 2 months. The hypervariable region V3 + V4 on 16S rDNA of intestinal bacterial flora was sequenced by the use of a high-flux sequencing system on a Miseq Illumina platform; then, the obtained sequences were analyzed. The results showed that, when compared with the control group, both genera and phyla of intestinal bacteria in the three treatment groups decreased. About one third of the total genera and one half of the total phyla remained in the high-dose group. The dominant flora were Lactobacillus and Bacteroides in all groups. There were significant differences in the relative abundance of intestinal bacterial flora among groups. Most bacteria decreased as a whole from the control to the high-dose groups, but several beneficial and pathogenic bacterial species increased significantly with increasing dose of AFB1. Thus, the conclusion was that intragastric feeding with 2.5~10 mg/mL AFB1 for 2 months could decrease the majority of intestinal bacterial flora and induce the proliferation of some intestinal bacteria flora. View Full-Text
Keywords: mice; aflatoxin B1; intestinal bacterial flora; response mice; aflatoxin B1; intestinal bacterial flora; response

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Yang, X.; Liu, L.; Chen, J.; Xiao, A. Response of Intestinal Bacterial Flora to the Long-term Feeding of Aflatoxin B1 (AFB1) in Mice. Toxins 2017, 9, 317.

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