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Aflatoxin B1-Induced Developmental and DNA Damage in Caenorhabditis elegans

Department of Environmental Health Science, College of Public Health, University of Georgia, Athens, GA 30602, USA
Wuxi Center for Disease Control and Prevention, Wuxi 214023, Jiangsu, China
Author to whom correspondence should be addressed.
Academic Editor: James J. Pestka
Received: 17 October 2016 / Revised: 6 December 2016 / Accepted: 22 December 2016 / Published: 26 December 2016
(This article belongs to the Collection Aflatoxins)
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Aflatoxin B1 (AFB1) is a ubiquitous mycotoxin produced by toxicogenic Aspergillus species. AFB1 has been reported to cause serious adverse health effects, such as cancers and abnormal development and reproduction, in animals and humans. AFB1 is also a potent genotoxic mutagen that causes DNA damage in vitro and in vivo. However, the link between DNA damage and abnormal development and reproduction is unclear. To address this issue, we examined the DNA damage, germline apoptosis, growth, and reproductive toxicity following exposure to AFB1, using Caenorhabditis elegans as a study model. Results found that AFB1 induced DNA damage and germline apoptosis, and significantly inhibited growth and reproduction of the nematodes in a concentration-dependent manner. Exposure to AFB1 inhibited growth or reproduction more potently in the DNA repair-deficient xpa-1 nematodes than the wild-type N2 strain. According to the relative expression level of pathway-related genes measured by real-time PCR, the DNA damage response (DDR) pathway was found to be associated with AFB1-induced germline apoptosis, which further played an essential role in the dysfunction of growth and reproduction in C. elegans. View Full-Text
Keywords: Aflatoxin B1; apoptosis; Caenorhabditis elegans; DNA damage response; growth and reproductive toxicity Aflatoxin B1; apoptosis; Caenorhabditis elegans; DNA damage response; growth and reproductive toxicity

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Feng, W.-H.; Xue, K.S.; Tang, L.; Williams, P.L.; Wang, J.-S. Aflatoxin B1-Induced Developmental and DNA Damage in Caenorhabditis elegans. Toxins 2017, 9, 9.

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