Detoxification of Aflatoxin B1 by Zygosaccharomyces rouxii with Solid State Fermentation in Peanut Meal
Abstract
:1. Introduction
2. Results
2.1. Screening Strains for the Detoxification of AFB1
2.2. Identification of Strain A
2.3. Effect of the Heat Temperature and Heat Time on AFB1 Detoxification
2.4. Removal of AFB1 in Liquid Fermentation
2.5. Verification of Detoxification of AFB1 by LC/MS
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Detoxification of Aflatoxins by Solid State Fermentation
5.1.1. Screening Strains for Detoxification of AFB1
5.1.2. Identification of Strain A
5.1.3. Effect of the Heating Temperature and Heating Time on AFB1 Detoxification
5.2. Removal of Aflatoxins by Cell and Cell-Free Liquid
5.3. Extraction of Residual AFB1 and HPLC Analysis
5.4. Verification of Detoxification of AFB1 by LC/MS
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Group | Source | Management Methods | AFB1 Residual Rate (%) (Mean ± Standard Deviation) |
---|---|---|---|
Untreated | 100 | ||
Strain A | fermented soy paste | 80 °C, pH 10, 10 min | 16.18 ± 0.2 |
Strain B | fermented soy paste | 80 °C, pH 10, 10 min | 36.68 ± 0.5 |
Strain C | fermented soy paste | 80 °C, pH 10, 10 min | 42.47 ± 0.1 |
Strain D | yoghourt | 80 °C, pH 10, 10 min | 37.67 ± 0.3 |
S. cerevisiae | Angel Company | 80 °C, pH 10, 10 min | 34.42 ± 0.5 |
B. megaterium | [23] | 80 °C, pH 10, 10 min | 36.46 ± 0.8 |
S. thermophilus | [21] | 80 °C, pH 10, 10 min | 17.63 ± 0.4 |
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Zhou, G.; Chen, Y.; Kong, Q.; Ma, Y.; Liu, Y. Detoxification of Aflatoxin B1 by Zygosaccharomyces rouxii with Solid State Fermentation in Peanut Meal. Toxins 2017, 9, 42. https://doi.org/10.3390/toxins9010042
Zhou G, Chen Y, Kong Q, Ma Y, Liu Y. Detoxification of Aflatoxin B1 by Zygosaccharomyces rouxii with Solid State Fermentation in Peanut Meal. Toxins. 2017; 9(1):42. https://doi.org/10.3390/toxins9010042
Chicago/Turabian StyleZhou, Guanghui, Yujie Chen, Qing Kong, Yunxiao Ma, and Yang Liu. 2017. "Detoxification of Aflatoxin B1 by Zygosaccharomyces rouxii with Solid State Fermentation in Peanut Meal" Toxins 9, no. 1: 42. https://doi.org/10.3390/toxins9010042
APA StyleZhou, G., Chen, Y., Kong, Q., Ma, Y., & Liu, Y. (2017). Detoxification of Aflatoxin B1 by Zygosaccharomyces rouxii with Solid State Fermentation in Peanut Meal. Toxins, 9(1), 42. https://doi.org/10.3390/toxins9010042