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Toxins 2016, 8(8), 233;

Selective Membrane Redistribution and Depletion of Gαq-Protein by Pasteurella multocida Toxin

Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
Current address: School of Biotechnology, Southern Medical University, Guangzhou 510515, China.
Author to whom correspondence should be addressed.
Academic Editor: Katharina Kubatzky
Received: 15 June 2016 / Revised: 22 July 2016 / Accepted: 25 July 2016 / Published: 1 August 2016
(This article belongs to the Special Issue Pasteurella multocida and Its Virulence Factors)
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Pasteurella multocida toxin (PMT), the major virulence factor responsible for zoonotic atrophic rhinitis, is a protein deamidase that activates the alpha subunit of heterotrimeric G proteins. Initial activation of G alpha-q-coupled phospholipase C-beta-1 signaling by PMT is followed by uncoupling of G alpha-q-dependent signaling, causing downregulation of downstream calcium and mitogenic signaling pathways. Here, we show that PMT decreases endogenous and exogenously expressed G alpha-q protein content in host cell plasma membranes and in detergent resistant membrane (DRM) fractions. This membrane depletion of G alpha-q protein was dependent upon the catalytic activity of PMT. Results indicate that PMT-modified G alpha-q redistributes within the host cell membrane from the DRM fraction into the soluble membrane and cytosolic fractions. In contrast, PMT had no affect on G alpha-s or G beta protein levels, which are not substrate targets of PMT. PMT also had no affect on G alpha-11 levels, even though G alpha-11 can serve as a substrate for deamidation by PMT, suggesting that membrane depletion of PMT-modified G-alpha-q has specificity. View Full-Text
Keywords: dermonecrosis; Pasteurella multocida; G-protein downregulation; mitogen; deamidation dermonecrosis; Pasteurella multocida; G-protein downregulation; mitogen; deamidation

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Clemons, N.C.; Luo, S.; Ho, M.; Wilson, B.A. Selective Membrane Redistribution and Depletion of Gαq-Protein by Pasteurella multocida Toxin. Toxins 2016, 8, 233.

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