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Selective Membrane Redistribution and Depletion of Gαq-Protein by Pasteurella multocida Toxin

Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
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Author to whom correspondence should be addressed.
Current address: School of Biotechnology, Southern Medical University, Guangzhou 510515, China.
Academic Editor: Katharina Kubatzky
Toxins 2016, 8(8), 233; https://doi.org/10.3390/toxins8080233
Received: 15 June 2016 / Revised: 22 July 2016 / Accepted: 25 July 2016 / Published: 1 August 2016
(This article belongs to the Special Issue Pasteurella multocida and Its Virulence Factors)
Pasteurella multocida toxin (PMT), the major virulence factor responsible for zoonotic atrophic rhinitis, is a protein deamidase that activates the alpha subunit of heterotrimeric G proteins. Initial activation of G alpha-q-coupled phospholipase C-beta-1 signaling by PMT is followed by uncoupling of G alpha-q-dependent signaling, causing downregulation of downstream calcium and mitogenic signaling pathways. Here, we show that PMT decreases endogenous and exogenously expressed G alpha-q protein content in host cell plasma membranes and in detergent resistant membrane (DRM) fractions. This membrane depletion of G alpha-q protein was dependent upon the catalytic activity of PMT. Results indicate that PMT-modified G alpha-q redistributes within the host cell membrane from the DRM fraction into the soluble membrane and cytosolic fractions. In contrast, PMT had no affect on G alpha-s or G beta protein levels, which are not substrate targets of PMT. PMT also had no affect on G alpha-11 levels, even though G alpha-11 can serve as a substrate for deamidation by PMT, suggesting that membrane depletion of PMT-modified G-alpha-q has specificity. View Full-Text
Keywords: dermonecrosis; Pasteurella multocida; G-protein downregulation; mitogen; deamidation dermonecrosis; Pasteurella multocida; G-protein downregulation; mitogen; deamidation
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MDPI and ACS Style

Clemons, N.C.; Luo, S.; Ho, M.; Wilson, B.A. Selective Membrane Redistribution and Depletion of Gαq-Protein by Pasteurella multocida Toxin. Toxins 2016, 8, 233.

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