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A P-Glycoprotein Is Linked to Resistance to the Bacillus thuringiensis Cry3Aa Toxin in a Leaf Beetle

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Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Str. 8, Jena 07745, Germany
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Unité de Zoologie Forestière, Institut National de la Recherche Agronomique (INRA), 2163 Avenue de la Pomme de Pin, CS 40001 Ardon, Orléans 45075 CEDEX 2, France
*
Author to whom correspondence should be addressed.
Academic Editors: Juan Ferré and Baltasar Escriche
Toxins 2016, 8(12), 362; https://doi.org/10.3390/toxins8120362
Received: 16 September 2016 / Revised: 21 November 2016 / Accepted: 25 November 2016 / Published: 5 December 2016
(This article belongs to the Special Issue The Insecticidal Bacterial Toxins in Modern Agriculture)
Chrysomela tremula is a polyvoltine oligophagous leaf beetle responsible for massive attacks on poplar trees. This beetle is an important model for understanding mechanisms of resistance to Bacillus thuringiensis (Bt) insecticidal toxins, because a resistant C. tremula strain has been found that can survive and reproduce on transgenic poplar trees expressing high levels of the Cry3Aa Bt toxin. Resistance to Cry3Aa in this strain is recessive and is controlled by a single autosomal locus. We used a larval midgut transcriptome for C. tremula to search for candidate resistance genes. We discovered a mutation in an ABC protein, member of the B subfamily homologous to P-glycoprotein, which is genetically linked to Cry3Aa resistance in C. tremula. Cultured insect cells heterologously expressing this ABC protein swell and lyse when incubated with Cry3Aa toxin. In light of previous findings in Lepidoptera implicating A subfamily ABC proteins as receptors for Cry2A toxins and C subfamily proteins as receptors for Cry1A and Cry1C toxins, this result suggests that ABC proteins may be targets of insecticidal three-domain Bt toxins in Coleoptera as well. View Full-Text
Keywords: ABC proteins; Bt Cry3Aa toxin; Chrysomela tremula; leaf beetle; Bt resistance ABC proteins; Bt Cry3Aa toxin; Chrysomela tremula; leaf beetle; Bt resistance
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Pauchet, Y.; Bretschneider, A.; Augustin, S.; Heckel, D.G. A P-Glycoprotein Is Linked to Resistance to the Bacillus thuringiensis Cry3Aa Toxin in a Leaf Beetle. Toxins 2016, 8, 362.

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