Toxins 2015, 7(9), 3740-3757; https://doi.org/10.3390/toxins7093740
First Report of Ciguatoxins in Two Starfish Species: Ophidiaster ophidianus and Marthasterias glacialis
Marisa Silva 1,2,†, Inés Rodriguez 3,†, Aldo Barreiro 1,2, Manfred Kaufmann 2,4,5
, Ana Isabel Neto 2,6, Meryem Hassouani 7, Brahim Sabour 7, Amparo Alfonso 3, Luis M. Botana 3 and Vitor Vasconcelos 1,2,*
, Ana Isabel Neto 2,6, Meryem Hassouani 7, Brahim Sabour 7, Amparo Alfonso 3, Luis M. Botana 3 and Vitor Vasconcelos 1,2,*
1
Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Porto 4619-007, Portugal
2
Interdisciplinary Center of Marine and Environmental Research—CIMAR/CIIMAR, University of Porto, Rua dos Bragas, 289, Porto 4050-123, Portugal
3
Department of Pharmacology, Faculty of Veterinary, University of Santiago of Compostela, Lugo 27002, Spain
4
Centre of Life Sciences, University of Madeira, Marine Biology Station of Funchal, Funchal 9000-107, Portugal
5
Center of Interdisciplinary Marine and Environmental Research of Madeira—CIIMAR-Madeira, Edif. Madeira Tecnopolo, Caminho da Penteada, Funchal 9020-105, Portugal
6
Department of Marine Biology, University of Azores, Ponta Delgada 9501-801, Portugal
7
Phycology Research Unit—Biotechnology, Ecosystems Ecology and Valorization Laboratory. Faculty of Sciences El Jadida, University Chouaib Doukkali, El Jadida BP20, Morocco
†
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Michio Murata
Received: 24 July 2015 / Revised: 15 September 2015 / Accepted: 16 September 2015 / Published: 21 September 2015
(This article belongs to the Section Marine and Freshwater Toxins)
Abstract
Ciguatera fish poisoning (CFP) is a syndrome caused by the ingestion of fish contaminated with Ciguatoxins (CTXs). These phycotoxins are produced mainly by dinoflagellates that belong to the genus Gambierdiscus that are transformed in more toxic forms in predatory fish guts, and are more present in the Indo-Pacific and Caribbean areas. It is estimated that CFP causes per year more than 10,000 intoxications worldwide. With the rise of water temperature and anthropogenic intervention, it is important to study the prevalence of CFP in more temperate waters. Through inter- and subtidal sampling, 22 species of organisms were collected, in Madeira and Azores archipelagos and in the northwestern Moroccan coast, during September of 2012 and June and July of 2013. A total of 94 samples of 22 different species of bivalves, gastropods, echinoderms and crustaceans where analyzed by Ultra Performance Liquid Chromatography-Mass Spectometry-Ion Trap-Time of Flight (UPLC-MS-IT-TOF) and Ultra Performance Chromatography- Mass Spectrometry (UPLC-MS). Our main aim was to detect new vectors and ascertain if there were some geographical differences. We detected for the first time putative CTXs in echinoderms, in two starfish species—M. glacialis and O. ophidianus. We detected differences regarding uptake values by organisms and geographical location. Toxin amounts were significant, showing the importance and the need for continuity of these studies to gain more knowledge about the prevalence of these toxins, in order to better access human health risk. In addition, we suggest monitoring of these toxins should be extended to other vectors, starfish being a good alternative for protecting and accessing human health risk. View Full-TextKeywords:
ciguatera; new vectors; Madeira Island; São Miguel Island; Morocco
▼
Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Share & Cite This Article
MDPI and ACS Style
Silva, M.; Rodriguez, I.; Barreiro, A.; Kaufmann, M.; Neto, A.I.; Hassouani, M.; Sabour, B.; Alfonso, A.; Botana, L.M.; Vasconcelos, V. First Report of Ciguatoxins in Two Starfish Species: Ophidiaster ophidianus and Marthasterias glacialis. Toxins 2015, 7, 3740-3757.