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Article

Functional Characterization of New Polyketide Synthase Genes Involved in Ochratoxin A Biosynthesis in Aspergillus Ochraceus fc-1

1
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, 1 Nongda South Road, Xibeiwang Town, Haidian District, Beijing 100193, China
2
Key Laboratory of Agro-products Processing, Ministry of Agriculture, 1 Nongda South Road, Xibeiwang Town, Haidian District, Beijing 100193, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Jiujiang Yu
Toxins 2015, 7(8), 2723-2738; https://doi.org/10.3390/toxins7082723
Received: 26 June 2015 / Revised: 10 July 2015 / Accepted: 17 July 2015 / Published: 24 July 2015
Ochratoxin A (OTA), a potentially carcinogenic mycotoxin which contaminates grains, is produced by several Aspergillus species. A comparative sequence analysis of the OTA-producing Aspergillus ochraceus fc-1 strain and other Aspergillus species was performed. Two new OTA-related polyketide synthase (PKS) (AoOTApks) genes were identified. The predicted amino acid sequence of AoOTApks-1 displayed high similarity to previously identified PKSs from OTA-producing A. carbonarius ITEM 5010 (67%; [PI] No. 173482) and A. niger CBS 513.88 (62%; XP_001397313). However, the predicted amino acid sequence of AoOTApks-2 displayed lower homology with A. niger CBS 513.88 (38%) and A. carbonarius ITEM 5010 (28%). A phylogenetic analysis of the β-ketosynthase and acyl-transferase domains of the AoOTApks proteins indicated that they shared a common origin with other OTA-producing species, such as A. carbonarius, A. niger, and A. westerdijkiae. A real-time reverse-transcription PCR analysis showed that the expression of AoOTApks-1 and -2 was positively correlated with the OTA concentration. The pks gene deleted mutants ∆AoOTApks-1 and ∆AoOTApks-2 produced nil and lesser OTA than the wild-type strain, respectively. Our study suggests that AoOTApks-1 could be involved in OTA biosynthesis, while AoOTApks-2 might be indirectly involved in OTA production. View Full-Text
Keywords: ochratoxin A; polyketide synthase; homologous recombination; biosynthetic pathway ochratoxin A; polyketide synthase; homologous recombination; biosynthetic pathway
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MDPI and ACS Style

Wang, L.; Wang, Y.; Wang, Q.; Liu, F.; Selvaraj, J.N.; Liu, L.; Xing, F.; Zhao, Y.; Zhou, L.; Liu, Y. Functional Characterization of New Polyketide Synthase Genes Involved in Ochratoxin A Biosynthesis in Aspergillus Ochraceus fc-1. Toxins 2015, 7, 2723-2738. https://doi.org/10.3390/toxins7082723

AMA Style

Wang L, Wang Y, Wang Q, Liu F, Selvaraj JN, Liu L, Xing F, Zhao Y, Zhou L, Liu Y. Functional Characterization of New Polyketide Synthase Genes Involved in Ochratoxin A Biosynthesis in Aspergillus Ochraceus fc-1. Toxins. 2015; 7(8):2723-2738. https://doi.org/10.3390/toxins7082723

Chicago/Turabian Style

Wang, Liuqing, Yan Wang, Qi Wang, Fei Liu, Jonathan N. Selvaraj, Lingna Liu, Fuguo Xing, Yueju Zhao, Lu Zhou, and Yang Liu. 2015. "Functional Characterization of New Polyketide Synthase Genes Involved in Ochratoxin A Biosynthesis in Aspergillus Ochraceus fc-1" Toxins 7, no. 8: 2723-2738. https://doi.org/10.3390/toxins7082723

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