Immunization of Mice with Anthrax Protective Antigen Limits Cardiotoxicity but Not Hepatotoxicity Following Lethal Toxin Challenge
Abstract
:1. Introduction
2. Results
2.2. Immunization with PA Induces Ab and Inhibits Cardiotoxicity but not Hepatotoxicity
2.3. Inclusion of Adjuvants in the PA Vaccine Does not Affect LT-Induced Hepatotoxicity
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Toxin Expression and Purification
4.3. Mice
4.4. Ethics Statement
4.5. Retro-Orbital Eye Bleed and Serum Collection
4.6. Immunizations and Experimental Schedule
4.7. In Vivo Toxin Challenge
4.8. ELISA for Serum Ig
4.9. AST, ALT, and cTn1 Measurement
4.10. Histological Analysis of Liver
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Devera, T.S.; Prusator, D.K.; Joshi, S.K.; Ballard, J.D.; Lang, M.L. Immunization of Mice with Anthrax Protective Antigen Limits Cardiotoxicity but Not Hepatotoxicity Following Lethal Toxin Challenge. Toxins 2015, 7, 2371-2384. https://doi.org/10.3390/toxins7072371
Devera TS, Prusator DK, Joshi SK, Ballard JD, Lang ML. Immunization of Mice with Anthrax Protective Antigen Limits Cardiotoxicity but Not Hepatotoxicity Following Lethal Toxin Challenge. Toxins. 2015; 7(7):2371-2384. https://doi.org/10.3390/toxins7072371
Chicago/Turabian StyleDevera, T. Scott, Dawn K. Prusator, Sunil K. Joshi, Jimmy D. Ballard, and Mark L. Lang. 2015. "Immunization of Mice with Anthrax Protective Antigen Limits Cardiotoxicity but Not Hepatotoxicity Following Lethal Toxin Challenge" Toxins 7, no. 7: 2371-2384. https://doi.org/10.3390/toxins7072371